ArticleThe EMBO journal2015
The Mediator subunit MED23 couples H2B mono-ubiquitination to transcriptional control and cell fate determination.
Article in The EMBO journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 38 citations in OpenAlex.
- The Mediator complex: co-transcriptional regulation and beyond.RNA biology · 2026Review
- Non-canonical subunit functions in multiprotein complexes: insights from the Mediator complex.Biochemical Society transactions · 2026Review
- Trans-histone crosstalk establishes distinct H3K79 methylation zones with differential transcriptional functions.Nucleic acids research · 2026Article
- Decoding RNF20: an epigenetic modifier and beyond.Frontiers in cell and developmental biology · 2026Review
- Structural basis of human Mediator recruitment by the phosphorylated transcription factor Elk-1.Nature communications · 2025Article
- The Mediator subunit OsMED23 associates with the histone demethylase OsJMJ703 and the transcription factor OsWOX3A to control grain size and yield in rice.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Mediator MED23 controls oligodendrogenesis and myelination by modulating Sp1/P300-directed gene programs.Cell discovery · 2024Article
- Article
- Article
- An intellectual disability-related MED23 mutation dysregulates gene expression by altering chromatin conformation and enhancer activities.Nucleic acids research · 2023Article
- SHP-1 dephosphorylates histone H2B to facilitate its ubiquitination during transcription.The EMBO journal · 2022Article
- Histone H2B ubiquitination mediated chromatin relaxation is essential for the induction of somatic cell reprogramming.Cell proliferation · 2021Article
- Med23 Regulates Sox9 Expression during Craniofacial Development.Journal of dental research · 2021Article
- Egr2-guided histone H2B monoubiquitination is required for peripheral nervous system myelination.Nucleic acids research · 2020Article
- Cdk9 and H2Bub1 signal to Clr6-CII/Rpd3S to suppress aberrant antisense transcription.Nucleic acids research · 2020Article
- Mediator Med23 Regulates Adult Hippocampal Neurogenesis.Frontiers in cell and developmental biology · 2020Article
- Mediator MED23 regulates inflammatory responses and liver fibrosis.PLoS biology · 2019Article
- Epigenetic regulation of ferroptosis by H2B monoubiquitination and p53.EMBO reports · 2019Article
- Novel mutation in theClinical case reports · 2019Article
- Med23 serves as a gatekeeper of the myeloid potential of hematopoietic stem cells.Nature communications · 2018Article
Corrections and comments
- Commented on by
Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
The Mediator complex orchestrates multiple transcription factors with the Pol II apparatus for precise transcriptional control. However, its interplay with the surrounding chromatin remains poorly understood. Here, we analyze differential histone modifications between WT and MED23(-/-) (KO) cells and identify H2B mono-ubiquitination at lysine 120 (H2Bub) as a MED23-dependent histone modification. Using tandem affinity purification and mass spectrometry, we find that MED23 associates with the RNF20/40 complex, the enzyme for H2Bub, and show that this association is critical for the recruitment of RNF20/40 to chromatin. In a cell-free system, Mediator directly and substantially increases H2Bub on recombinant chromatin through its cooperation with RNF20/40 and the PAF complex. Integrative genome-wide analyses show that MED23 depletion specifically reduces H2Bub on a subset of MED23-controlled genes. Importantly, MED23-coupled H2Bub levels are oppositely regulated during myogenesis and lung carcinogenesis. In sum, these results establish a mechanistic link between the Mediator complex and a critical chromatin modification in coordinating transcription with cell growth and differentiation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.