ArticleScientific reports2015
Rapid, high efficiency isolation of pancreatic ß-cells.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- BODIPY-Based Molecules for Biomedical Applications.Biomolecules · 2023Review
- Targeting β Cells with Cathelicidin Nanomedicines Improves Insulin Function and Pancreas Regeneration in Type 1 Diabetic Rats.ACS pharmacology & translational science · 2023Article
- Revealing the tissue-level complexity of endogenous glucagon-like peptide-1 receptor expression and signaling.Nature communications · 2023Article
- Reagents and models for detecting endogenous GLP1R and GIPR.EBioMedicine · 2021Review
- Isolation and Proteomics of the Insulin Secretory Granule.Metabolites · 2021Review
- Revisiting the Complexity of GLP-1 Action from Sites of Synthesis to Receptor Activation.Endocrine reviews · 2021Review
- Roles of oxidative stress, apoptosis, and inflammation in metal-induced dysfunction of beta pancreatic cells isolated from CD1 mice.Saudi journal of biological sciences · 2021Article
- Allograft inflammatory factor-1 in myeloid cells drives autoimmunity in type 1 diabetes.JCI insight · 2020Article
- Super-resolution microscopy compatible fluorescent probes reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics.Nature communications · 2020Article
- Navigating the Depths and Avoiding the Shallows of Pancreatic Islet Cell Transcriptomes.Diabetes · 2019Article
- Assessment of antipsychotic-induced cytotoxic effects on isolated CD1 mouse pancreatic beta cells.International journal of health sciencesArticle
- Cyclic AMP and calcium signaling are involved in antipsychotic-induced diabetogenic effects in isolated pancreatic β cells of CD1 mice.International journal of health sciencesArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
The ability to isolate pure pancreatic ß-cells would greatly aid multiple areas of diabetes research. We developed a fluorescent exendin-4-like neopeptide conjugate for the rapid purification and isolation of functional mouse pancreatic β-cells. By targeting the glucagon-like peptide-1 receptor with the fluorescent conjugate, β-cells could be quickly isolated by flow cytometry and were >99% insulin positive. These studies were confirmed by immunostaining, microscopy and gene expression profiling on isolated cells. Gene expression profiling studies of cytofluorometrically sorted β-cells from 4 and 12 week old NOD mice provided new insights into the genetic programs at play of different stages of type-1 diabetes development. The described isolation method should have broad applicability to the β-cell field.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.