ArticleJournal of lipid research2015
LipiD-QuanT: a novel method to quantify lipid accumulation in live cells.
Article in Journal of lipid research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Label-free measurements of lipid droplets in cells based on quantitative differential phase contrast imaging with high oblique illumination.Biomedical optics express · 2026Article
- Lipid droplet size profiling in yeast.Biology open · 2026Article
- Wnt/β-catenin signaling activation promotes lipogenesis in the steatotic liver via physical mTOR interaction.Frontiers in endocrinology · 2023Article
- Protein and lipid mass concentration measurement in tissues by stimulated Raman scattering microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Macrophage-derived secretome is sufficient to confer olanzapine-mediated insulin resistance in human adipocytes.Comprehensive psychoneuroendocrinology · 2021Article
- Systemic NF-κB-mediated inflammation promotes an aging phenotype in skeletal stem/progenitor cells.Aging · 2021Article
- Neutral lipids as early biomarkers of cellular fate: the case of α-synuclein overexpression.Cell death & disease · 2021Article
- Comparison of Cell-Free Extracts from Three Newly IdentifiedBioMed research international · 2021Article
- Melatonin reduces intramuscular fat deposition by promoting lipolysis and increasing mitochondrial function.Journal of lipid research · 2019Article
- Quantitative imaging of lipid droplets in single cells.The Analyst · 2019Review
- Fast Adipogenesis Tracking System (FATS)-a robust, high-throughput, automation-ready adipogenesis quantification technique.Stem cell research & therapy · 2019Article
- Focal Adhesion Kinase and ROCK Signaling Are Switch-Like Regulators of Human Adipose Stem Cell Differentiation towards Osteogenic and Adipogenic Lineages.Stem cells international · 2018Article
- Evidence of a DHA Signature in the Lipidome and Metabolome of Human Hepatocytes.International journal of molecular sciences · 2017Article
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4 authors.
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Abstract
Lipid droplets (LDs) are the main storage organelles for triglycerides. Elucidation of lipid accumulation mechanisms and metabolism are essential to understand obesity and associated diseases. Adipogenesis has been well studied in murine 3T3-L1 and human Simpson-Golabi-Behmel syndrome (SGBS) preadipocyte cell lines. However, most techniques for measuring LD accumulation are either not quantitative or can be destructive to samples. Here, we describe a novel, label-free LD quantification technique (LipiD-QuanT) to monitor lipid dynamics based on automated image analysis of phase contrast microscopy images acquired during in vitro human adipogenesis. We have applied LipiD-QuanT to measure LD accumulation during differentiation of SGBS cells. We demonstrate that LipiD-QuanT is a robust, nondestructive, time- and cost-effective method compared with other triglyceride accumulation assays based on enzymatic digest or lipophilic staining. Further, we applied LipiD-QuanT to measure the effect of four potential pro- or antiobesogenic substances: DHA, rosiglitazone, elevated levels of D-glucose, and zinc oxide nanoparticles. Our results revealed that 2 µmol/l rosiglitazone treatment during adipogenesis reduced lipid production and caused a negative shift in LD diameter size distribution, but the other treatments showed no effect under the conditions used here.
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