ArticleParasites & vectors2015
Development of novel multiplex microsatellite polymerase chain reactions to enable high-throughput population genetic studies of Schistosoma haematobium.
Article in Parasites & vectors, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Potential drivers for schistosomiasis persistence: Population genetic analyses from a cluster-randomized urogenital schistosomiasis elimination trial across the Zanzibar islands.PLoS neglected tropical diseases · 2022Trial
- The research contribution of the Schistosomiasis Collection at the Natural History Museum (SCAN): highlights, challenges and future directions.Infectious diseases of poverty · 2025Review
- Population genetics and molecular xenomonitoring of Biomphalaria freshwater snails along the southern shoreline of Lake Malawi, Malawi.Parasites & vectors · 2024Article
- African schistosomes in small mammal communities: Perspectives from a spatio-temporal survey in the vicinity of Lake Guiers, Senegal.PLoS neglected tropical diseases · 2024Article
- Molecular epidemiology and population genetics of Schistosoma mansoni infecting school-aged children situated along the southern shoreline of Lake Malawi, Malawi.PLoS neglected tropical diseases · 2024Article
- Genetic characterization of schistosome species from cattle in Côte d'Ivoire.Parasites & vectors · 2024Article
- Population genetic structure of Schistosoma bovis and S. curassoni collected from cattle in Mali.Parasite (Paris, France) · 2024Article
- Molecular characterization of schistosome cercariae and theirCurrent research in parasitology & vector-borne diseases · 2023Article
- Transmission and diversity of Schistosoma haematobium and S. bovis and their freshwater intermediate snail hosts Bulinus globosus and B. nasutus in the Zanzibar Archipelago, United Republic of Tanzania.PLoS neglected tropical diseases · 2022Article
- Population Genetic Structure and Hybridization ofPathogens (Basel, Switzerland) · 2022Article
- Hybridization increases genetic diversity in Schistosoma haematobium populations infecting humans in Cameroon.Infectious diseases of poverty · 2022Article
- Population genetic structure of Schistosoma haematobium and Schistosoma haematobium × Schistosoma bovis hybrids among school-aged children in Côte d'Ivoire.Parasite (Paris, France) · 2022Article
- Membrane Technology for Rapid Point-of-Care Diagnostics for Parasitic Neglected Tropical Diseases.Clinical microbiology reviews · 2021Article
- Identification of Snails andFrontiers in public health · 2021Article
- Schistosomiasis Morbidity Hotspots: Roles of the Human Host, the Parasite and Their Interface in the Development of Severe Morbidity.Frontiers in immunology · 2021Review
- The detection ofCurrent research in parasitology & vector-borne diseases · 2021Article
- Prevalence and distribution of schistosomiasis in human, livestock, and snail populations in northern Senegal: a One Health epidemiological study of a multi-host system.The Lancet. Planetary health · 2020Article
- Parasite Population Genetic Contributions to the Schistosomiasis Consortium for Operational Research and Evaluation within Sub-Saharan Africa.The American journal of tropical medicine and hygiene · 2020Review
- Multihost Transmission of Schistosoma mansoni in Senegal, 2015-2018.Emerging infectious diseases · 2020Article
- Interactions between Schistosoma haematobium group species and their Bulinus spp. intermediate hosts along the Niger River Valley.Parasites & vectors · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
13 authors.
Funding
Abstract
backgroundHuman urogenital schistosomiasis caused by Schistosoma haematobium is widely distributed across Africa and is increasingly targeted for control and regional elimination. The development of new high-throughput, cost-effective molecular tools and approaches are needed to monitor and evaluate the impact of control programs on the parasite populations. Microsatellite loci are genetic markers that can be used to investigate how parasite populations change over time and in relation to external influences such as control interventions.
findingsHere, 18 existing S. haematobium microsatellite loci were optimised to enable simultaneous amplification across two novel multiplex microsatellite PCR's, each containing nine loci. Methods were developed for the cost effective and rapid processing and microsatellite analysis of S. haematobium larval stages stored on Whatman-FTA cards and proved robust on miracidia and cercariae collected from Zanzibar and Niger.
conclusionThe development of these novel and robust multiplex microsatellite assays, in combination with an improved protocol to elute gDNA from Whatman-FTA fixed schistosome larval stages, enables the high-throughput population genetic analysis of S. haematobium. The molecular resources and protocols described here advance the way researchers can perform multi locus-based population genetic analyses of S. haematobium as part of the evaluation and monitoring of schistosomiasis control programmes.
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