ArticleBMC biology2015
Genome-wide replication landscape of Candida glabrata.
Article in BMC biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 18 citations in OpenAlex.
- Chromosome-scale assembly of the streamlined picoeukaryoteMicrobial genomics · 2024Article
- Article
- Abf1 Is an Essential Protein That Participates in Cell Cycle Progression and Subtelomeric Silencing inJournal of fungi (Basel, Switzerland) · 2021Article
- Conservation of a DNA Replication Motif among Phylogenetically Distant Budding Yeast Species.Genome biology and evolution · 2021Article
- Orc4 spatiotemporally stabilizes centromeric chromatin.Genome research · 2021Article
- Identification of Essential Genes and Fluconazole Susceptibility Genes inG3 (Bethesda, Md.) · 2020Article
- Spatial inter-centromeric interactions facilitated the emergence of evolutionary new centromeres.eLife · 2020Article
- The evolution of the temporal program of genome replication.Nature communications · 2018Article
- DNA replication timing influences gene expression level.The Journal of cell biology · 2017Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe opportunistic pathogen Candida glabrata is a member of the Saccharomycetaceae yeasts. Like its close relative Saccharomyces cerevisiae, it underwent a whole-genome duplication followed by an extensive loss of genes. Its genome contains a large number of very long tandem repeats, called megasatellites. In order to determine the whole replication program of the C. glabrata genome and its general chromosomal organization, we used deep-sequencing and chromosome conformation capture experiments.
resultsWe identified 253 replication fork origins, genome wide. Centromeres, HML and HMR loci, and most histone genes are replicated early, whereas natural chromosomal breakpoints are located in late-replicating regions. In addition, 275 autonomously replicating sequences (ARS) were identified during ARS-capture experiments, and their relative fitness was determined during growth competition. Analysis of ARSs allowed us to identify a 17-bp consensus, similar to the S. cerevisiae ARS consensus sequence but slightly more constrained. Megasatellites are not in close proximity to replication origins or termini. Using chromosome conformation capture, we also show that early origins tend to cluster whereas non-subtelomeric megasatellites do not cluster in the yeast nucleus.
conclusionsDespite a shorter cell cycle, the C. glabrata replication program shares unexpected striking similarities to S. cerevisiae, in spite of their large evolutionary distance and the presence of highly repetitive large tandem repeats in C. glabrata. No correlation could be found between the replication program and megasatellites, suggesting that their formation and propagation might not be directly caused by replication fork initiation or termination.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.