ArticleScientific reports2015
PEA-CLARITY: 3D molecular imaging of whole plant organs.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 83 citations in OpenAlex.
- Super-resolution expansion microscopy in plant roots.The Plant cell · 2025Article
- A Protocol for Live Imaging of Arabidopsis Gynoecium Development Using Confocal Microscopy.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Clearing techniques for deeper imaging of plants and plant-microbe interactions.Applied microscopy · 2024Review
- Elevated nocturnal respiratory rates in the mitochondria of CAM plants: current knowledge and unanswered questions.Annals of botany · 2023Article
- Comparing the efficiency of six clearing methods in developing seeds of Arabidopsis thaliana.Plant reproduction · 2022Article
- 3D multiple immunoimaging using whole male organs in rice.Scientific reports · 2022Article
- Flip-Flap: A Simple Dual-View Imaging Method for 3D Reconstruction of Thick Plant Samples.Plants (Basel, Switzerland) · 2022Article
- Improved clearing method contributes to deep imaging of plant organs.Communications biology · 2022Article
- Measuring Plasmodesmata Density on Cell Interfaces of Monocot Leaves Using 3D Immunolocalization and Scanning Electron Microscopy.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Whole-Tissue Three-Dimensional Imaging of Rice at Single-Cell Resolution.International journal of molecular sciences · 2021Article
- Tissue clearing and 3D imaging in developmental biology.Development (Cambridge, England) · 2021Review
- Basic principles of hydrogel-based tissue transformation technologies and their applications.Cell · 2021Review
- Physical and chemical mechanisms of tissue optical clearing.iScience · 2021Review
- Action potentials induce biomagnetic fields in carnivorous Venus flytrap plants.Scientific reports · 2021Article
- Direct fluorescence imaging of lignocellulosic and suberized cell walls in roots and stems.AoB PLANTS · 2020Article
- Whole-Brain Profiling of Cells and Circuits in Mammals by Tissue Clearing and Light-Sheet Microscopy.Neuron · 2020Review
- Fluorescent reporters for functional analysis in rice leaves.Plant direct · 2020Article
- Genetic Screens to Target Embryo and Endosperm Pathways in Arabidopsis and Maize.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Probing the 3D architecture of the plant nucleus with microscopy approaches: challenges and solutions.Nucleus (Austin, Tex.) · 2019Review
- Systems models, phenomics and genomics: three pillars for developing high-yielding photosynthetically efficient crops.In silico plants · 2019Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here we report the adaptation of the CLARITY technique to plant tissues with addition of enzymatic degradation to improve optical clearing and facilitate antibody probe penetration. Plant-Enzyme-Assisted (PEA)-CLARITY, has allowed deep optical visualisation of stains, expressed fluorescent proteins and IgG-antibodies in Tobacco and Arabidopsis leaves. Enzyme treatment enabled penetration of antibodies into whole tissues without the need for any sectioning of the material, thus facilitating protein localisation of intact tissue in 3D whilst retaining cellular structure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.