ArticleGastroenterology and hepatology from bed to bench2015
Multilocus variable-number tandem-repeat analysis for genotyping of Shigella sonnei strains isolated from pediatric patients.
Article in Gastroenterology and hepatology from bed to bench, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Molecular characterization and antimicrobial resistance profiles of Shigella flexneri isolates from pediatric clinical cases in Ahvaz, Iran.Scientific reports · 2026Article
- Virulence factors and molecular characteristics of Shigella flexneri isolated from calves with diarrhea.BMC microbiology · 2021Article
- Distribution of genes encoding virulence factors of Shigella strains isolated from children with diarrhea in southwest Iran.Molecular biology reports · 2021Article
- Multilocus Variable-Number Tandem-Repeat Analysis for Geno-typing ofIranian journal of public health · 2020Article
- Frequency and molecular epidemiology of class A ESBLs producing EnteroinvasiveGastroenterology and hepatology from bed to bench · 2020Article
- Distribution of Genes Encoding Virulence Factors and the Genetic Diversity of EnteroinvasiveInfection and drug resistance · 2020Article
- Detection of Plasmid-MediatedThe open microbiology journal · 2018Article
- Virulence Gene Profile and Multilocus Variable-Number Tandem-Repeat Analysis (MLVA) of Enteroinvasive Escherichia coli (EIEC) Isolates From Patients With Diarrhea in Kerman, Iran.Jundishapur journal of microbiology · 2016Article
- Molecular diagnosis and anti-microbial resistance patterns among Shigella spp. isolated from patients with diarrhea.Gastroenterology and hepatology from bed to bench · 2016Article
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Authors and funding
2 authors.
Funding
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Abstract
aimThe aims of this study were to characterize Iranian Shigella sonnei strains isolated from pediatric cases and evaluate the utility of multilocus variable-number tandem-repeat (VNTR) analysis (MLVA) for genotyping of local S. sonnei strains.
backgroundS. sonnei has become the dominant species in certain parts of Iran. Although PFGE is still a gold standard for genotyping and source tracking of food-borne pathogens, it is laborious, expensive, time-consuming, and often difficult to interpret. However, MLVA is a PCR-based method, which is rapid, relatively inexpensive and easy to perform. PATIENTS AND
methodsA total of 47 S. sonnei isolates were obtained from sporadic cases of pediatric shigellosis in Tehran, Iran, during the years 2002-2003 (n=10) and 2008-2010 (n=37). The patients suffered from acute diarrhea and had evidence of more than three episodes of watery, loose, or bloody stools per day. A MLVA scheme based on 7 VNTR loci was established to assess the diversity of 47 S. sonnei isolates.
resultsBased on the results, it was clear that the S. sonnei isolates were heterogeneous. Overall, 47 S. sonnei isolates were discriminated into 21 different genotypes. Analysis of the MLVA profiles using a minimum spanning tree (MST) algorithm showed the usefulness of the MLVA assay in discriminating S. sonnei isolates collected over different time periods. However, no correlation was found between the MLVA genotypes and age, gender or clinical symptoms of the patients.
conclusionIt is assumed that our S. sonnei isolates are derived from a limited number of clones that undergo minor genetic changes in the course of time. The present study has provided some valuable insights into the genetic relatedness of S. sonnei in Tehran, Iran.
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