ArticleBiochimica et biophysica acta2015
Defining the effects of storage on platelet bioenergetics: The role of increased proton leak.
Article in Biochimica et biophysica acta, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 34 citations in OpenAlex.
- Integrative metabolomics and transcriptomics signatures of clinical tolerance to Plasmodium vivax reveal activation of innate cell immunity and T cell signaling.Redox biology · 2018Trial
- Buyang Huanwu decoction ameliorates myocardial injury and attenuates platelet activation by regulating the PI3 kinase/Rap1/integrin α(IIb)β(3) pathway.Chinese medicine · 2024Article
- Metabolism of parathyroid organoids.Frontiers in endocrinology · 2023Article
- Platelets' morphology, metabolic profile, exocytosis, and heterotypic aggregation with leukocytes in relation to severity and mortality of COVID-19-patients.Frontiers in immunology · 2022Article
- The program of renal fibrogenesis is controlled by microRNAs regulating oxidative metabolism.Redox biology · 2021Article
- Long-term treated HIV infection is associated with platelet mitochondrial dysfunction.Scientific reports · 2021Article
- Differential Leukocyte and Platelet Profiles in Distinct Models of Traumatic Brain Injury.Cells · 2021Article
- Article
- Computational Identification and Characterization of New microRNAs in Human Platelets Stored in a Blood Bank.Biomolecules · 2020Article
- Mitochondrial damage and senescence phenotype of cells derived from a novel frataxin G127V point mutation mouse model of Friedreich's ataxia.Disease models & mechanisms · 2020Article
- A precision medicine approach to defining the impact of doxorubicin on the bioenergetic-metabolite interactome in human platelets.Redox biology · 2020Article
- MicroRNAs as a Potential Quality Measurement Tool of Platelet Concentrate Stored in Blood Banks-A Review.Cells · 2019Review
- Feasibility of cellular bioenergetics as a biomarker in porphyria patients.Molecular genetics and metabolism reports · 2019Article
- Mitochondria in precision medicine; linking bioenergetics and metabolomics in platelets.Redox biology · 2019Article
- Platelet biomechanics, platelet bioenergetics, and applications to clinical practice and translational research.Platelets · 2018Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
The quality of platelets decreases over storage time, shortening their shelf life and potentially worsening transfusion outcomes. The changes in mitochondrial function associated with platelet storage are poorly defined and to address this we measured platelet bioenergetics in freshly isolated and stored platelets. We demonstrate that the hypotonic stress test stimulates both glycolysis and oxidative phosphorylation and the stored platelets showed a decreased recovery to this stress. We found no change in aggregability between the freshly isolated and stored platelets. Bioenergetic parameters were changed including increased proton leak and decreased basal respiration and this was reflected in a lower bioenergetic health index (BHI). Mitochondrial electron transport, measured in permeabilized platelets, showed only minor changes which are unlikely to have a significant impact on platelet function. There were no changes in basal glycolysis between the fresh and stored platelets, however, glycolytic rate was increased in stored platelets when mitochondrial ATP production was inhibited. The increase in proton leak was attenuated by the addition of albumin, suggesting that free fatty acids could play a role in increasing proton leak and decreasing mitochondrial function. In summary, platelet storage causes a modest decrease in oxidative phosphorylation driven by an increase in mitochondrial proton leak, which contributes to the decreased recovery to hypotonic stress.
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