ArticlePLoS neglected tropical diseases2015
Analysis of Dengue Virus Genetic Diversity during Human and Mosquito Infection Reveals Genetic Constraints.
Article in PLoS neglected tropical diseases, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 63 citations in OpenAlex.
- Extended Evaluation of Virological, Immunological and Pharmacokinetic Endpoints of CELADEN: A Randomized, Placebo-Controlled Trial of Celgosivir in Dengue Fever Patients.PLoS neglected tropical diseases · 2016Trial
- Intrahost population dynamics of chikungunya virus in humans and naturally infectedVirus evolution · 2026Article
- Exploring the genetic diversity of the 3' untranslated region (UTR) of Dengue virus.Virology journal · 2025Article
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- Dengue virus surveillance in Nepal yields the first on-site whole genome sequences of isolates from the 2022 outbreak.BMC genomics · 2024Article
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- Retrospective Genomic Characterization of a 2017 Dengue Virus Outbreak, Burkina Faso.Emerging infectious diseases · 2022Article
- Intra-Host Diversity of Dengue Virus in Mosquito Vectors.Frontiers in cellular and infection microbiology · 2022Review
- Flavivirus Persistence in Wildlife Populations.Viruses · 2021Review
- Cluster of Symptomatic Graft-to-Host Transmission of Herpes Simplex Virus Type 1 in an Endothelial Keratoplasty Setting.Ophthalmology science · 2021Article
- Attenuated dengue viruses are genetically more diverse than their respective wild-type parents.NPJ vaccines · 2021Article
- Genetic Variation in the Domain II, 3' Untranslated Region of Human and Mosquito Derived Dengue Virus Strains in Sri Lanka.Viruses · 2021Article
- Effect of low-passage number on dengue consensus genomes and intra-host variant frequencies.The Journal of general virology · 2021Article
- Full-Length Genome and Partial Viral Genes Phylogenetic and Geographical Analysis of Dengue Serotype 3 Isolates.Microorganisms · 2021Article
- Principles of dengue virus evolvability derived from genotype-fitness maps in human and mosquito cells.eLife · 2021Article
- Epidemiological and genetic aspects of the largest dengue outbreak recorded in 2015 in Southeastern Brazil.Revista do Instituto de Medicina Tropical de Sao Paulo · 2021Article
- Alternation between taxonomically divergent hosts is not the major determinant of flavivirus evolution.Virus evolution · 2021Article
- Within-Host Viral Diversity: A Window into Viral Evolution.Annual review of virology · 2020Review
- Whole genome sequencing and genetic variations in several dengue virus type 1 strains from unusual dengue epidemic of 2017 in Vietnam.Virology journal · 2020Article
- Application of Next-Generation Sequencing to Reveal How Evolutionary Dynamics of Viral Population Shape Dengue Epidemiology.Frontiers in microbiology · 2020Review
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dengue viruses (DENV) cause debilitating and potentially life-threatening acute disease throughout the tropical world. While drug development efforts are underway, there are concerns that resistant strains will emerge rapidly. Indeed, antiviral drugs that target even conserved regions in other RNA viruses lose efficacy over time as the virus mutates. Here, we sought to determine if there are regions in the DENV genome that are not only evolutionarily conserved but genetically constrained in their ability to mutate and could hence serve as better antiviral targets. High-throughput sequencing of DENV-1 genome directly from twelve, paired dengue patients' sera and then passaging these sera into the two primary mosquito vectors showed consistent and distinct sequence changes during infection. In particular, two residues in the NS5 protein coding sequence appear to be specifically acquired during infection in Ae. aegypti but not Ae. albopictus. Importantly, we identified a region within the NS3 protein coding sequence that is refractory to mutation during human and mosquito infection. Collectively, these findings provide fresh insights into antiviral targets and could serve as an approach to defining evolutionarily constrained regions for therapeutic targeting in other RNA viruses.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.