ArticlePLoS neglected tropical diseases2015
Neutrophil Extracellular Traps Identification in Tegumentary Lesions of Patients with Paracoccidioidomycosis and Different Patterns of NETs Generation In Vitro.
Article in PLoS neglected tropical diseases, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Paracoccidioidomycosis in the 21st century: Challenges and milestones.PLoS neglected tropical diseases · 2026Review
- Pulmonary Hypertension Secondary to Fungal Infections: Underexplored Pathological Links.Infectious disease reports · 2025Article
- Antifungal Innate Immunity.Advances in experimental medicine and biology · 2025Review
- The toxic effects of neutrophil extracellular traps on mesenchymal stem cells.Molecular biology reports · 2024Article
- Review
- The DermatophyteJournal of fungi (Basel, Switzerland) · 2022Article
- The emerging role of neutrophil extracellular traps in fungal infection.Frontiers in cellular and infection microbiology · 2022Review
- Pathogen-Derived Nucleases: An Effective Weapon for Escaping Extracellular Traps.Frontiers in immunology · 2022Review
- Neutrophil and Eosinophil DNA Extracellular Trap Formation: Lessons From Pathogenic Fungi.Frontiers in microbiology · 2021Review
- The Role of the Interleukin-17 Axis and Neutrophils in the Pathogenesis of Endemic and Systemic Mycoses.Frontiers in cellular and infection microbiology · 2020Review
- Article
- Neutrophil extracellular traps in fungal infection.Seminars in cell & developmental biology · 2019Review
- 43 kDa Glycoprotein (gp43) fromJournal of immunology research · 2019Article
- CD40 ligand deficiency causes functional defects of peripheral neutrophils that are improved by exogenous IFN-γ.The Journal of allergy and clinical immunology · 2018Article
- Article
- Human neutrophils are targets to paracoccin, a lectin expressed by Paracoccidioides brasiliensis.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2018Article
- Platelets: an outlook from biology through evidence-based achievements in critical care.Annals of translational medicine · 2017Review
- Evaluation of the in vitro and in vivo proinflammatory activities of gold (+) and gold (-) nanoparticles.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2017Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Paracoccidioidomycosis (PCM) is a systemic mycosis, endemic in most Latin American countries, especially in Brazil. It is caused by the thermo-dimorphic fungus of the genus Paracoccidioides (Paracoccidioides brasiliensis and Paracoccidioides lutzii). Innate immune response plays a crucial role in host defense against fungal infections, and neutrophils (PMNs) are able to combat microorganisms with three different mechanisms: phagocytosis, secretion of granular proteins, which have antimicrobial properties, and the most recent described mechanism called NETosis. This new process is characterized by the release of net-like structures called Neutrophil Extracellular Traps (NETs), which is composed of nuclear (decondensed DNA and histones) and granular material such as elastase. Several microorganisms have the ability of inducing NETs formation, including gram-positive and gram-negative bacteria, viruses and some fungi. We proposed to identify NETs in tegumentary lesions of patients with PCM and to analyze the interaction between two strains of P. brasiliensis and human PMNs by NETs formation in vitro. In this context, the presence of NETs in vivo was evidenced in tegumentary lesions of patients with PCM by confocal spectrum analyzer. Furthermore, we showed that the high virulent P. brasiliensis strain 18 (Pb18) and the lower virulent strain Pb265 are able to induce different patterns of NETs formation in vitro. The quantification of extracellular DNA corroborates the idea of the ability of P. brasiliensis in inducing NETs release. In conclusion, our data show for the first time the identification of NETs in lesions of patients with PCM and demonstrate distinct patterns of NETs in cultures challenged with fungi in vitro. The presence of NETs components both in vivo and in vitro open new possibilities for the detailed investigation of immunity in PCM.
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