ArticleLangmuir : the ACS journal of surfaces and colloids2015
High-Throughput Universal DNA Curtain Arrays for Single-Molecule Fluorescence Imaging.
Article in Langmuir : the ACS journal of surfaces and colloids, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Single-molecule DNA flow-stretch assays for high-throughput DNA-protein interaction studies.FEBS open bio · 2026Article
- Mechanism of Cas9 inhibition by AcrIIA11.Nucleic acids research · 2025Article
- Probing the effect of PEG-DNA interactions and buffer viscosity on tethered DNA in shear flow.bioRxiv : the preprint server for biology · 2025Article
- Single-molecule DNA-flow stretching assay as a versatile hybrid tool for investigating DNA-protein interactions.BMB reports · 2025Review
- Probing the effect of PEG-DNA interactions and buffer viscosity on tethered DNA in shear flow.PloS one · 2025Article
- Sperm chromatin structure and reproductive fitness are altered by substitution of a single amino acid in mouse protamine 1.Nature structural & molecular biology · 2023Article
- smFRET Detection of Cis and Trans DNA Interactions by the BfiI Restriction Endonuclease.The journal of physical chemistry. B · 2023Article
- Micropatterning of functional lipid bilayer assays for quantitative bioanalysis.Biomicrofluidics · 2023Article
- The MRN complex and topoisomerase IIIa-RMI1/2 synchronize DNA resection motor proteins.The Journal of biological chemistry · 2023Article
- Single Molecule Imaging of DNA-Protein Interactions Using DNA Curtains.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Polymerase theta-helicase promotes end joining by stripping single-stranded DNA-binding proteins and bridging DNA ends.Nucleic acids research · 2022Article
- Construction of a Three-Color Prism-Based TIRF Microscope to Study the Interactions and Dynamics of Macromolecules.Biology · 2021Article
- Molecular basis of the dual role of the Mlh1-Mlh3 endonuclease in MMR and in meiotic crossover formation.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
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- Oriented Soft DNA Curtains for Single-Molecule Imaging.Langmuir : the ACS journal of surfaces and colloids · 2021Article
- HP1 proteins compact DNA into mechanically and positionally stable phase separated domains.eLife · 2021Article
- DNA Manipulation and Single-Molecule Imaging.Molecules (Basel, Switzerland) · 2021Review
- Epigenetic cell fate in Candida albicans is controlled by transcription factor condensates acting at super-enhancer-like elements.Nature microbiology · 2020Article
- RADX condenses single-stranded DNA to antagonize RAD51 loading.Nucleic acids research · 2020Article
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Authors and funding
7 authors.
Funding
Abstract
Single-molecule studies of protein-DNA interactions have shed critical insights into the molecular mechanisms of nearly every aspect of DNA metabolism. The development of DNA curtains-a method for organizing arrays of DNA molecules on a fluid lipid bilayer-has greatly facilitated these studies by increasing the number of reactions that can be observed in a single experiment. However, the utility of DNA curtains is limited by the challenges associated with depositing nanometer-scale lipid diffusion barriers onto quartz microscope slides. Here, we describe a UV lithography-based method for large-scale fabrication of chromium (Cr) features and organization of DNA molecules at these features for high-throughput single-molecule studies. We demonstrate this approach by assembling 792 independent DNA arrays (containing >900,000 DNA molecules) within a single microfluidic flowcell. As a first proof of principle, we track the diffusion of Mlh1-Mlh3-a heterodimeric complex that participates in DNA mismatch repair and meiotic recombination. To further highlight the utility of this approach, we demonstrate a two-lane flowcell that facilitates concurrent experiments on different DNA substrates. Our technique greatly reduces the challenges associated with assembling DNA curtains and paves the way for the rapid acquisition of large statistical data sets from individual single-molecule experiments.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.