ArticleProceedings of the National Academy of Sciences of the United States of America2015
Complexity of the human memory B-cell compartment is determined by the versatility of clonal diversification in germinal centers.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 65 citations in OpenAlex.
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- Differential immune profiles in elderly patients with non-severe versus severe SARS-CoV-2 omicron variant infection: dysregulation of antibody responses and B-cell subsets.Immunity & ageing : I & A · 2025Article
- IgMScientific reports · 2025Article
- B Cell Differentiation and the Origin and Pathogenesis of Human B Cell Lymphomas.Methods in molecular biology (Clifton, N.J.) · 2025Review
- B cell diversification in gut-associated lymphoid tissues: From birds to humans.The Journal of experimental medicine · 2023Article
- Human IgMInternational journal of molecular sciences · 2023Article
- CD27 on human memory B cells-more than just a surface marker.Clinical and experimental immunology · 2023Review
- T-independent responses to polysaccharides in humans mobilize marginal zone B cells prediversified against gut bacterial antigens.Science immunology · 2023Article
- Clonal relationships of memory B cell subsets in autoimmune mice.Frontiers in immunology · 2023Article
- Clonal composition and differentiation stage of human CD30Frontiers in immunology · 2023Article
- Amount of antigen, T follicular helper cells and affinity of founder cells shape the diversity of germinal center B cells: A computational study.Frontiers in immunology · 2023Article
- Human B-cell subset identification and changes in inflammatory diseases.Clinical and experimental immunology · 2022Review
- B cells in human lymphoid structures.Clinical and experimental immunology · 2022Review
- Memory B cells and long-lived plasma cells in AMR.Renal failure · 2022Review
- Comprehensive phenotyping of human peripheral blood B lymphocytes in healthy conditions.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2022Review
- Comprehensive phenotyping of human peripheral blood B lymphocytes in pathological conditions.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2022Article
- Allogeneic haematopoietic stem cell transplantation resets T- and B-cell compartments in sickle cell disease patients.Clinical & translational immunology · 2022Article
- Optimized Protocols forFrontiers in immunology · 2022Article
- Double-negative (DN) B cells: an under-recognized effector memory B cell subset in autoimmunity.Clinical and experimental immunology · 2021Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Our knowledge about the clonal composition and intraclonal diversity of the human memory B-cell compartment and the relationship between memory B-cell subsets is still limited, although these are central issues for our understanding of adaptive immunity. We performed a deep sequencing analysis of rearranged immunoglobulin (Ig) heavy chain genes from biological replicates, covering more than 100,000 memory B lymphocytes from two healthy adults. We reveal a highly similar B-cell receptor repertoire among the four main human IgM(+) and IgG(+) memory B-cell subsets. Strikingly, in both donors, 45% of sequences could be assigned to expanded clones, demonstrating that the human memory B-cell compartment is characterized by many, often very large, B-cell clones. Twenty percent of the clones consisted of class switched and IgM(+)(IgD(+)) members, a feature that correlated significantly with clone size. Hence, we provide strong evidence that the vast majority of Ig mutated B cells--including IgM(+)IgD(+)CD27(+) B cells--are post-germinal center (GC) memory B cells. Clone members showed high intraclonal sequence diversity and high intraclonal versatility in Ig class and IgG subclass composition, with particular patterns of memory B-cell clone generation in GC reactions. In conclusion, GC produce amazingly large, complex, and diverse memory B-cell clones, equipping the human immune system with a versatile and highly diverse compartment of IgM(+)(IgD(+)) and class-switched memory B cells.
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