ArticleProceedings of the National Academy of Sciences of the United States of America2015
Discovery of a novel amino acid racemase through exploration of natural variation in Arabidopsis thaliana.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 62 citations in OpenAlex.
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- Structure and inhibition of diaminopimelic acid epimerase by slow-binding α-methyl amino acids.Protein science : a publication of the Protein Society · 2025Article
- Genetic basis and selection of glyceollin elicitation in wild soybean.Frontiers in plant science · 2024Article
- Plant biochemical genetics in the multiomics era.Journal of experimental botany · 2023Review
- Review
- QT-GWAS: A novel method for unveiling biosynthetic loci affecting qualitative metabolic traits.Molecular plant · 2023Article
- A 21-bp InDel in the promoter ofHorticulture research · 2023Article
- Genome-wide association mapping reveals genes underlying population-level metabolome diversity in a fungal crop pathogen.BMC biology · 2022Article
- Using interdisciplinary, phylogeny-guided approaches to understand the evolution of plant metabolism.Plant molecular biology · 2022Review
- Metabolic source isotopic pair labeling and genome-wide association are complementary tools for the identification of metabolite-gene associations in plants.The Plant cell · 2021Article
- Ancestral sequence reconstruction - An underused approach to understand the evolution of gene function in plants?Computational and structural biotechnology journal · 2021Review
- Natural Variation in Plant Pluripotency and Regeneration.Plants (Basel, Switzerland) · 2020Review
- Review
- AraPheno and the AraGWAS Catalog 2020: a major database update including RNA-Seq and knockout mutation data for Arabidopsis thaliana.Nucleic acids research · 2020Article
- Using Local Convolutional Neural Networks for Genomic Prediction.Frontiers in genetics · 2020Article
- AtDAT1 Is a Key Enzyme of D-Amino Acid Stimulated Ethylene Production inFrontiers in plant science · 2019Article
- Bioprospecting Plant Growth-Promoting Rhizobacteria That Mitigate Drought Stress in Grasses.Frontiers in microbiology · 2019Article
- Asexual Female Gametogenesis Involves Contact with a Sexually-Fated Megaspore in ApomicticPlant physiology · 2018Article
- Discovering variation of secondary metabolite diversity and its relationship with disease resistance inEcology and evolution · 2018Article
- Comprehensive transcriptome analyses correlated with untargeted metabolome reveal differentially expressed pathways in response to cell wall alterations.Plant molecular biology · 2018Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plants produce diverse low-molecular-weight compounds via specialized metabolism. Discovery of the pathways underlying production of these metabolites is an important challenge for harnessing the huge chemical diversity and catalytic potential in the plant kingdom for human uses, but this effort is often encumbered by the necessity to initially identify compounds of interest or purify a catalyst involved in their synthesis. As an alternative approach, we have performed untargeted metabolite profiling and genome-wide association analysis on 440 natural accessions of Arabidopsis thaliana. This approach allowed us to establish genetic linkages between metabolites and genes. Investigation of one of the metabolite-gene associations led to the identification of N-malonyl-D-allo-isoleucine, and the discovery of a novel amino acid racemase involved in its biosynthesis. This finding provides, to our knowledge, the first functional characterization of a eukaryotic member of a large and widely conserved phenazine biosynthesis protein PhzF-like protein family. Unlike most of known eukaryotic amino acid racemases, the newly discovered enzyme does not require pyridoxal 5'-phosphate for its activity. This study thus identifies a new d-amino acid racemase gene family and advances our knowledge of plant d-amino acid metabolism that is currently largely unexplored. It also demonstrates that exploitation of natural metabolic variation by integrating metabolomics with genome-wide association is a powerful approach for functional genomics study of specialized metabolism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.