ArticleThe Journal of biological chemistry2015
Crystal Structure of the Minor Pilin CofB, the Initiator of CFA/III Pilus Assembly in Enterotoxigenic Escherichia coli.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 20 citations in OpenAlex.
- Insight into the relationship between type IV pilus function and biofilm formation.Biochemical Society transactions · 2026Review
- Type IV pili ofEcoSal Plus · 2024Review
- Mechanism of secretion of TcpF by theProceedings of the National Academy of Sciences of the United States of America · 2023Article
- The minor pilin PilV provides a conserved adhesion site throughout the antigenically variable meningococcal type IV pilus.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- PilB fromProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Structure and function of minor pilins of type IV pili.Medical microbiology and immunology · 2020Review
- TheThe Journal of biological chemistry · 2019Article
- Type IV pili: dynamics, biophysics and functional consequences.Nature reviews. Microbiology · 2019Review
- Article
- DNA Uptake by Type IV Filaments.Frontiers in molecular biosciences · 2019Review
- Gram-Positive Type IV Pili and Competence.Microbiology spectrum · 2019Article
- Interplay of a secreted protein with type IVb pilus for efficient enterotoxigenicProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Architecture of the Vibrio cholerae toxin-coregulated pilus machine revealed by electron cryotomography.Nature microbiology · 2017Article
- Motility and adhesion through type IV pili in Gram-positive bacteria.Biochemical Society transactions · 2016Review
- The Vibrio cholerae Minor Pilin TcpB Initiates Assembly and Retraction of the Toxin-Coregulated Pilus.PLoS pathogens · 2016Article
- Effects of tcpB Mutations on Biogenesis and Function of the Toxin-Coregulated Pilus, the Type IVb Pilus of Vibrio cholerae.Journal of bacteriology · 2016Article
- Effects of lng Mutations on LngA Expression, Processing, and CS21 Assembly in Enterotoxigenic Escherichia coli E9034A.Frontiers in microbiology · 2016Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Type IV pili are extracellular polymers of the major pilin subunit. These subunits are held together in the pilus filament by hydrophobic interactions among their N-terminal α-helices, which also anchor the pilin subunits in the inner membrane prior to pilus assembly. Type IV pilus assembly involves a conserved group of proteins that span the envelope of Gram-negative bacteria. Among these is a set of minor pilins, so named because they share their hydrophobic N-terminal polymerization/membrane anchor segment with the major pilins but are much less abundant. Minor pilins influence pilus assembly and retraction, but their precise functions are not well defined. The Type IV pilus systems of enterotoxigenic Escherichia coli and Vibrio cholerae are among the simplest of Type IV pilus systems and possess only a single minor pilin. Here we show that the enterotoxigenic E. coli minor pilins CofB and LngB are required for assembly of their respective Type IV pili, CFA/III and Longus. Low levels of the minor pilins are optimal for pilus assembly, and CofB can be detected in the pilus fraction. We solved the 2.0 Å crystal structure of N-terminally truncated CofB, revealing a pilin-like protein with an extended C-terminal region composed of two discrete domains connected by flexible linkers. The C-terminal region is required for CofB to initiate pilus assembly. We propose a model for CofB-initiated pilus assembly with implications for understanding filament growth in more complex Type IV pilus systems as well as the related Type II secretion system.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.