Evidence map›Paper›PMID 26324435›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2015

Fluorescent In Situ Hybridization of Nuclear Bodies in Drosophila melanogaster Ovaries.

Zehra F Nizami, Ji-Long Liu, Joseph G Gall

Open access · greenAbstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact, top 95% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Science advances · 2023
    Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 3 institutions in 2 countries.

Zehra F NizamiDepartment of Embryology, Carnegie Institution for Science, Baltimore, MD, 21218, USA.
Ji-Long Liu
Joseph G Gall
Carnegie Institution for Science · USDepartment of Embryology · USUniversity of Oxford · GB

Funding

THE ORGANIZATION OF ANIMAL CELL NUCLEIR01GM033397 · NIGMS · CARNEGIE INSTITUTION OF WASHINGTON, D.C. · PI GALL, JOSEPH G. · 1985 to 2019
$8.4M
NIGMS NIH HHS R01 GM033397
6 · The paper itself

Abstract

Fluorescent in situ hybridization (FISH) is a technique for determining the cytological localization of RNA or DNA molecules. There are many approaches available for generating in situ hybridization probes and conducting the subsequent hybridization steps. Here, we describe a simple and reliable FISH method to label small RNAs (200-500 nucleotides in length) that are enriched in nuclear bodies in Drosophila melanogaster ovaries, such as Cajal bodies (CBs) and histone locus bodies (HLBs). This technique can also be applied to other Drosophila tissues, and to abundant mRNAs such as histone transcripts.

Indexed as

AnimalsCoiled BodiesDrosophila melanogasterEmbryo, NonmammalianFemaleHistonesIn Situ Hybridization, FluorescenceOvaryRNA, MessengerHistonesRNA, Messenger

Identifiers

PMID26324435
PMCPMC5588913
OpenAlexW1243944199

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.