Evidence map›Paper›PMID 26324265›Full record

ArticleAntimicrobial agents and chemotherapy2015

Functions of a GyrBA fusion protein and its interaction with QnrB and quinolones.

Chunhui Chen, Regis Villet, George A Jacoby, David C Hooper

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Antimicrobial agents and chemotherapy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Chunhui ChenDivision of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA Institute of Antibiotics, Huashan Hospital, Fudan University, and Key Laboratory of Clinical Pharmacology of Antibiotics, National Health and Family Planning Commission, Shanghai, China.
Regis VilletDivision of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA.
George A JacobyLahey Hospital and Medical Center, Burlington, Massachusetts, USA.
David C HooperDivision of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA dhooper@mgh.harvard.edu.

Funding

Plasmid-mediated Quinolone ResistanceR01AI057576 · NIAID · MASSACHUSETTS GENERAL HOSPITAL · PI HOOPER, DAVID C · 2004 to 2018
$5.3M
NIAID NIH HHS R01 AI057576
6 · The paper itself

Abstract

In order to study the interactions between Escherichia coli DNA gyrase and the gyrase interacting protein QnrB in vivo, we constructed a gyrB-gyrA fusion and validated its ability to correct the temperature-sensitive growth of gyrA and gyrB mutants. Like wild-type gyrA, the gyrB-gyrA fusion complemented a quinolone-resistant gyrA mutant to increase susceptibility. It functioned as an active type II topoisomerase, catalyzed negative supercoiling of DNA, was inhibited by quinolone, and was protected by QnrB.

Indexed as

Anti-Infective AgentsDNA, BacterialDNA GyraseEscherichia coliEscherichia coli ProteinsQuinolonesRecombinant Fusion ProteinsAnti-Infective AgentsDNA, BacterialDNA GyraseEscherichia coli ProteinsQuinolonesRecombinant Fusion Proteins

Identifiers

PMID26324265
PMCPMC4604398

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.