ArticleEuropean journal of nuclear medicine and molecular imaging2016
Quantification of TSPO overexpression in a rat model of local neuroinflammation induced by intracerebral injection of LPS by the use of [(18)F]DPA-714 PET.
Article in European journal of nuclear medicine and molecular imaging, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 29 citations in OpenAlex.
- TSPO activation ameliorates maternal immune activation induced PV interneuron deficits via BDNF/TrkB signaling.Psychopharmacology · 2025Article
- TSPO imaging in animal models of brain diseases.European journal of nuclear medicine and molecular imaging · 2021Review
- Resolving the cellular specificity of TSPO imaging in a rat model of peripherally-induced neuroinflammation.Brain, behavior, and immunity · 2021Article
- Recent developments on PET radiotracers for TSPO and their applications in neuroimaging.Acta pharmaceutica Sinica. B · 2021Review
- TSPO Versus P2X7 as a Target for Neuroinflammation: An In Vitro and In Vivo Study.Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2020Article
- PET Imaging of the P2X7 Ion Channel with a Novel Tracer [Molecular imaging and biology · 2019Article
- Volume-of-interest-based supervised cluster analysis for pseudo-reference region selection in [EJNMMI research · 2018Article
- Letter to the Editor re: Increased Expression of Translocator Protein (TSPO) Marks Pro-inflammatory Microglia but Does Not Predict Neurodegeneration.Molecular imaging and biology · 2018Article
- Increased Expression of Translocator Protein (TSPO) Marks Pro-inflammatory Microglia but Does Not Predict Neurodegeneration.Molecular imaging and biology · 2018Article
- Reconceptualization of translocator protein as a biomarker of neuroinflammation in psychiatry.Molecular psychiatry · 2018Review
- Comparative Evaluation of Three TSPO PET Radiotracers in a LPS-Induced Model of Mild Neuroinflammation in Rats.Molecular imaging and biology · 2017Article
- Role of neuro-immunological factors in the pathophysiology of mood disorders.Psychopharmacology · 2016Review
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purpose[(18)F]DPA-714 is a radiotracer with high affinity for TSPO. We have characterized the kinetics of [(18)F]DPA-714 in rat brain and evaluated its ability to quantify TSPO expression with PET using a neuroinflammation model induced by unilateral intracerebral injection of lipopolysaccharide (LPS).
methodsDynamic small-animal PET scans with [(18)F]DPA-714 were performed in Wistar rats on a FOCUS-220 system for up to 3 h. Both plasma and perfused brain homogenates were analysed using HPLC to quantify radiometabolites. Full kinetic modelling of [(18)F]DPA-714 brain uptake was performed using a metabolite-corrected arterial plasma input function. Binding potential (BPND) calculated as the distribution volume ratio minus one (DVR-1) between affected and healthy brain tissue was used as the outcome measure and evaluated against reference tissue models.
resultsThe percentage of intact [(18)F]DPA-714 in arterial plasma samples was 92 ± 4 % at 10 min, 75 ± 8 % at 40 min and 52 ± 6 % at 180 min. The radiometabolite fraction in brain was negligible (<3 % at 30 min). Among the models investigated, the reversible two-tissue (2T) compartment model best described [(18)F]DPA-714 brain kinetics. BPND values obtained with a simplified and a multilinear reference tissue model (SRTM, MRTM) using the contralateral striatum as the reference region correlated well (Spearman's r = 0.96, p ≤ 0.003) with 2T BPND values calculated as DVR-1, and showed comparable bias (bias range 17.94 %, 20.32 %). Analysis of stability over time suggested that the acquisition time should be at least 90 min for SRTM and MRTM.
conclusionQuantification of [(18)F]DPA-714 binding to TSPO with full kinetic modelling is feasible using a 2T model. SRTM and MRTM can be suggested as reasonable substitutes with the contralateral striatum as the reference region and a scan duration of at least 90 min. However, selection of the reference region depends on the disease model used.
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