ArticleScientific reports2015
Silica bioreplication preserves three-dimensional spheroid structures of human pluripotent stem cells and HepG2 cells.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- The Role of Confinement in Biomineralization.Chemical reviews · 2025Review
- Synthetic thermoresponsive scaffolds for the expansion and differentiation of human pluripotent stem cells into cardiomyocytes.RSC advances · 2025Article
- Insights into spheroids formation in cellulose nanofibrils and Matrigel hydrogels using AFM-based techniques.Materials today. Bio · 2024Article
- Synthetic Thermo-Responsive Terpolymers as Tunable Scaffolds for Cell Culture Applications.Polymers · 2022Article
- Arrested in Glass: Actin within Sophisticated Architectures of Biosilica in Sponges.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Size-controlled human adipose-derived stem cell spheroids hybridized with single-segmented nanofibers and their effect on viability and stem cell differentiation.Biomaterials research · 2021Article
- Novel Breast Cancer Brain Metastasis Patient-Derived Orthotopic Xenograft Model for Preclinical Studies.Cancers · 2020Article
- Quantified forces between HepG2 hepatocarcinoma and WA07 pluripotent stem cells with natural biomaterials correlate with in vitro cell behavior.Scientific reports · 2019Article
- Hepatocyte Growth Factor: A Microenvironmental Resource for Leukemic Cell Growth.International journal of molecular sciences · 2019Review
- Bioinstructive microparticles for self-assembly of mesenchymal stem Cell-3D tumor spheroids.Biomaterials · 2018Article
- Multifunctional nanomedicine with silica: Role of silica in nanoparticles for theranostic, imaging, and drug monitoring.Journal of colloid and interface science · 2018Review
- Bioinspired Silicification Reveals Structural Detail in Self-Assembled Peptide Cages.ACS nano · 2018Article
- In Vitro Growth of Human Keratinocytes and Oral Cancer Cells into Microtissues: An Aerosol-Based Microencapsulation Technique.Bioengineering (Basel, Switzerland) · 2017Article
- Effects of Culture Dimensions on Maintenance of Porcine Inner Cell Mass-Derived Cell Self-Renewal.Molecules and cells · 2017Article
- Nanofibrillar cellulose-alginate hydrogel coated surgical sutures as cell-carrier systems.PloS one · 2017Article
- Composites of Polymer Hydrogels and Nanoparticulate Systems for Biomedical and Pharmaceutical Applications.Nanomaterials (Basel, Switzerland) · 2015Review
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three-dimensional (3D) cell cultures produce more in vivo-like multicellular structures such as spheroids that cannot be obtained in two-dimensional (2D) cell cultures. Thus, they are increasingly employed as models for cancer and drug research, as well as tissue engineering. It has proven challenging to stabilize spheroid architectures for detailed morphological examination. Here we overcome this issue using a silica bioreplication (SBR) process employed on spheroids formed from human pluripotent stem cells (hPSCs) and hepatocellular carcinoma HepG2 cells cultured in the nanofibrillar cellulose (NFC) hydrogel. The cells in the spheroids are more round and tightly interacting with each other than those in 2D cultures, and they develop microvilli-like structures on the cell membranes as seen in 2D cultures. Furthermore, SBR preserves extracellular matrix-like materials and cellular proteins. These findings provide the first evidence of intact hPSC spheroid architectures and similar fine structures to 2D-cultured cells, providing a pathway to enable our understanding of morphogenesis in 3D cultures.
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