ArticleAsian-Australasian journal of animal sciences2015
RNA-seq Profiles of Immune Related Genes in the Spleen of Necrotic Enteritis-afflicted Chicken Lines.
Article in Asian-Australasian journal of animal sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 53 citations in OpenAlex.
- Identification of immune-associated genes with altered expression in the spleen of mice enriched with probiotic Lactobacillus species using RNA-seq profiling.Animal bioscience · 2025Article
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- Identification of MicroRNAs RegulatingCurrent issues in molecular biology · 2023Article
- Change in Long Non-Coding RNA Expression Profile Related to the Antagonistic Effect ofCurrent issues in molecular biology · 2023Article
- Baicalin Alleviates Short-Term Lincomycin-Induced Intestinal and Liver Injury and Inflammation in Infant Mice.International journal of molecular sciences · 2022Article
- TMT labeled comparative proteomic analysis reveals spleen active immune responses duringPeerJ · 2022Article
- Transcriptomic Analysis of Laying Hens Revealed the Role of Aging-Related Genes during Forced Molting.Genes · 2021Article
- Quantitative analysis of the blood transcriptome of young healthy pigs and its relationship with subsequent disease resilience.BMC genomics · 2021Article
- Transcriptomic analysis of the liver in aged laying hens with different intensity of brown eggshell color.Animal bioscience · 2021Article
- Growth hormone receptor disrupts glucose homeostasis via promoting and stabilizing retinol binding protein 4.Theranostics · 2021Article
- Transcriptome analysis of genes related to gonad differentiation and development in Muscovy ducks.BMC genomics · 2020Article
- Effects of Bacillus cereus PAS38 on Immune-Related Differentially Expressed Genes of Spleen in Broilers.Probiotics and antimicrobial proteins · 2020Article
- Characterization and functional analyses of novel chicken leukocyte immunoglobulin-like receptor subfamily B members 4 and 5.Poultry science · 2019Article
- Eimeria maxima-induced transcriptional changes in the cecal mucosa of broiler chickens.Parasites & vectors · 2019Article
- Chicken novel leukocyte immunoglobulin-like receptor subfamilies B1 and B3 are transcriptional regulators of major histocompatibility complex class I genes and signaling pathways.Asian-Australasian journal of animal sciences · 2019Article
- Deciphering desirable immune responses from disease models with resistant and susceptible chickens.Poultry science · 2019Review
- Transcriptome analysis of adipose tissue from pigs divergent in feed efficiency reveals alteration in gene networks related to adipose growth, lipid metabolism, extracellular matrix, and immune response.Molecular genetics and genomics : MGG · 2019Article
- Novel Insights reveal Anti-microbial Gene Regulation of Piglet Intestine Immune in response to Clostridium perfringens Infection.Scientific reports · 2019Article
- Article
- Protective Effect of Ginsenoside Rg1 on Oxidative Damage Induced by Hydrogen Peroxide in Chicken Splenic Lymphocytes.Oxidative medicine and cellular longevity · 2019Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The study aimed to compare the necrotic enteritis (NE)-induced transcriptome differences between the spleens of Marek's disease resistant chicken line 6.3 and susceptible line 7.2 co-infected with Eimeria maxima/Clostridium perfringens using RNA-Seq. Total RNA from the spleens of two chicken lines were used to make libraries, generating 42,736,296 and 42,617,720 usable reads, which were assembled into groups of 29,897 and 29,833 mRNA genes, respectively. The transcriptome changes were investigated using the differentially expressed genes (DEGs) package, which indicated 3,255, 2,468 and 2,234 DEGs of line 6.3, line 7.2, and comparison between two lines, respectively (fold change ≥2, p<0.01). The transcription levels of 14 genes identified were further examined using qRT-PCR. The results of qRT-PCR were consistent with the RNA-seq data. All of the DEGs were analysed using gene ontology terms, the Kyoto Encyclopedia of Genes and Genomes (KEGG) database and the DEGs in each term were found to be more highly expressed in line 6.3 than in line 7.2. RNA-seq analysis indicated 139 immune related genes, 44 CD molecular genes and 150 cytokines genes which were differentially expressed among chicken lines 6.3 and 7.2 (fold change ≥2, p<0.01). Novel mRNA analysis indicated 15,518 novel genes, for which the expression was shown to be higher in line 6.3 than in line 7.2 including some immune-related targets. These findings will help to understand host-pathogen interaction in the spleen and elucidate the mechanism of host genetic control of NE, and provide basis for future studies that can lead to the development of marker-based selection of highly disease-resistant chickens.
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