ArticleJournal of lipid research2015
Metabolic engineering of Pichia pastoris to produce ricinoleic acid, a hydroxy fatty acid of industrial importance.
Article in Journal of lipid research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 49 citations in OpenAlex.
- Efficient production of itaconic acid from the single-carbon substrate methanol with engineered Komagataella phaffii.Biotechnology for biofuels and bioproducts · 2024Article
- Microbial Cell Factories in the Bioeconomy Era: From Discovery to Creation.Biodesign research · 2024Review
- Methanol-based biomanufacturing of fuels and chemicals using native and synthetic methylotrophs.Synthetic and systems biotechnology · 2023Article
- EngineeringSynthetic and systems biotechnology · 2023Article
- Plasmid-Based Gene Knockout Strategy with Subsequent Marker Recycling in Pichia pastoris.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Screening and evaluation of the strong endogenous promoters in Pichia pastoris.Microbial cell factories · 2021Article
- Co-expressingMetabolic engineering communications · 2021Article
- Functional Analysis of an Acyltransferase-Like Domain from Polyunsaturated Fatty Acid Synthase in Thraustochytrium.Microorganisms · 2021Article
- Metabolic Engineering for Unusual Lipid Production inMicroorganisms · 2020Review
- CRISPR-Cas9-mediated genomic multiloci integration in Pichia pastoris.Microbial cell factories · 2019Article
- Metabolic Engineering for Enhanced Medium Chain Omega Hydroxy Fatty Acid Production inFrontiers in microbiology · 2018Article
- Three, two, one yeast fatty acid desaturases: regulation and function.World journal of microbiology & biotechnology · 2017Review
- Production of ricinoleic acid-containing monoestolide triacylglycerides in an oleaginous diatom, Chaetoceros gracilis.Scientific reports · 2016Article
- Novel homologous lactate transporter improves L-lactic acid production from glycerol in recombinant strains of Pichia pastoris.Microbial cell factories · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ricinoleic acid (12-hydroxyoctadec-cis-9-enoic acid) has many specialized uses in bioproduct industries, while castor bean is currently the only commercial source for the fatty acid. This report describes metabolic engineering of a microbial system (Pichia pastoris) to produce ricinoleic acid using a "push" (synthesis) and "pull" (assembly) strategy. CpFAH, a fatty acid hydroxylase from Claviceps purpurea, was used for synthesis of ricinoleic acid, and CpDGAT1, a diacylglycerol acyl transferase for the triacylglycerol synthesis from the same species, was used for assembly of the fatty acid. Coexpression of CpFAH and CpDGAT1 produced higher lipid contents and ricinoleic acid levels than expression of CpFAH alone. Coexpression in a mutant haploid strain defective in the Δ12 desaturase activity resulted in a higher level of ricinoleic acid than that in the diploid strain. Intriguingly, the ricinoleic acid produced was mainly distributed in the neutral lipid fractions, particularly the free fatty acid form, but with little in the polar lipids. This work demonstrates the effectiveness of the metabolic engineering strategy and excellent capacity of the microbial system for production of ricinoleic acid as an alternative to plant sources for industrial uses.
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