ArticleRNA (New York, N.Y.)2015
Dual functions of Macpiwi1 in transposon silencing and stem cell maintenance in the flatworm Macrostomum lignano.
Article in RNA (New York, N.Y.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- One probe fits all: a highly customizable modular RNA in situ hybridization platform expanding the application of SABER DNA probes.Development (Cambridge, England) · 2025Article
- Optimized protocols for RNA interference in Macrostomum lignano.G3 (Bethesda, Md.) · 2024Article
- piRNA generation is associated with the pioneer round of translation in stem cells.Nucleic acids research · 2024Article
- The microbiome of the marine flatworm Macrostomum lignano provides fitness advantages and exhibits circadian rhythmicity.Communications biology · 2023Article
- Article
- Transposon control as a checkpoint for tissue regeneration.Development (Cambridge, England) · 2022Article
- A pan-metazoan concept for adult stem cells: the wobbling Penrose landscape.Biological reviews of the Cambridge Philosophical Society · 2022Article
- A Review of Discovery Profiling of PIWI-Interacting RNAs and Their Diverse Functions in Metazoans.International journal of molecular sciences · 2021Review
- Lost and Found: Piwi and Argonaute Pathways in Flatworms.Frontiers in cellular and infection microbiology · 2021Article
- Somatic piRNAs and Transposons are Differentially Expressed Coincident with Skeletal Muscle Atrophy and Programmed Cell Death.Frontiers in genetics · 2021Article
- piRNA-Guided Genome Defense: From Biogenesis to Silencing.Annual review of genetics · 2018Review
- Metagenomic sequencing suggests a diversity of RNA interference-like responses to viruses across multicellular eukaryotes.PLoS genetics · 2018Article
- Conservation and diversification of small RNA pathways within flatworms.BMC evolutionary biology · 2017Article
- Histone-derived piRNA biogenesis depends on the ping-pong partners Piwi5 and Ago3 in Aedes aegypti.Nucleic acids research · 2017Article
- Article
- Vertically- and horizontally-transmitted memories - the fading boundaries between regeneration and inheritance in planaria.Biology open · 2016Review
- Adhesive organ regeneration in Macrostomum lignano.BMC developmental biology · 2016Article
- Genome and transcriptome of the regeneration-competent flatworm, Macrostomum lignano.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
Abstract
PIWI proteins and piRNA pathways are essential for transposon silencing and some aspects of gene regulation during animal germline development. In contrast to most animal species, some flatworms also express PIWIs and piRNAs in somatic stem cells, where they are required for tissue renewal and regeneration. Here, we have identified and characterized piRNAs and PIWI proteins in the emerging model flatworm Macrostomum lignano. We found that M. lignano encodes at least three PIWI proteins. One of these, Macpiwi1, acts as a key component of the canonical piRNA pathway in the germline and in somatic stem cells. Knockdown of Macpiwi1 dramatically reduces piRNA levels, derepresses transposons, and severely impacts stem cell maintenance. Knockdown of the piRNA biogenesis factor Macvasa caused an even greater reduction in piRNA levels with a corresponding increase in transposons. Yet, in Macvasa knockdown animals, we detected no major impact on stem cell self-renewal. These results may suggest stem cell maintenance functions of PIWI proteins in flatworms that are distinguishable from their impact on transposons and that might function independently of what are considered canonical piRNA populations.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.