ArticleNature nanotechnology2015
High-throughput optical sensing of nucleic acids in a nanopore array.
Article in Nature nanotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
60 citing papers in PubMed.
- Engineered MspA Nanopores for Real-Time Detection of Insulin, Its Pharmaceutical Analogues, and Degradation Products.ACS nano · 2026Article
- Structural basis for independent pore function of Vpb4 from Bacillus thuringiensis.Nature communications · 2026Article
- Interrogating nanopores with light: optipore sensing for single molecule analyses.Journal of nanobiotechnology · 2026Review
- Metrology of Nanoscale Objects Using Nanopores.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Optical and Electrical Characterization of Nanopores in Freestanding Lipid Bilayers Formed on Microelectrode Cavity Arrays.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Heterogeneous Nanopore Arrays - Selective Modification of Nanopores Embedded in a Membrane.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Understanding single-molecule reactions using nanopore-based techniques.Nature chemistry · 2025Review
- Tracing the evolution of sequencing into the era of genomic medicine.Nature reviews. Genetics · 2025Review
- Advancing synthesis-free and enzyme-free rewritable DNA memory through frameshift encoding and nanopore duplex interruption decoding.PNAS nexus · 2025Article
- Neuromorphic ionic computing in droplet interface synapses.Science advances · 2025Article
- Tracking the Activity and Position of Mitochondrial β-Barrel Proteins.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Selective Single-Molecule Nanopore Detection of mpox A29 Protein Directly in Biofluids.Nano letters · 2023Article
- Confirming Silent Translocation through Nanopores with Simultaneous Single-Molecule Fluorescence and Single-Channel Electrical Recordings.Analytical chemistry · 2023Article
- The ER calcium channel Csg2 integrates sphingolipid metabolism with autophagy.Nature communications · 2023Article
- High-resolution discrimination of homologous and isomeric proteinogenic amino acids in nanopore sensors with ultrashort single-walled carbon nanotubes.Nature communications · 2023Article
- Digital and Absolute Assays for Low Abundance Molecular Biomarkers.Accounts of chemical research · 2023Article
- An Engineered OmpG Nanopore with Displayed Peptide Motifs for Single-Molecule Multiplex Protein Detection.Angewandte Chemie (International ed. in English) · 2023Article
- Single-molecule tracking of perfringolysin O assembly and membrane insertion uncoupling.The FEBS journal · 2023Article
- Building programmable multicompartment artificial cells incorporating remotely activated protein channels using microfluidics and acoustic levitation.Nature communications · 2022Article
- Encapsulated droplet interface bilayers as a platform for high-throughput membrane studies.Soft matter · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Protein nanopores such as α-haemolysin and Mycobacterium smegmatis porin A (MspA) can be used to sequence long strands of DNA at low cost. To provide high-speed sequencing, large arrays of nanopores are required, but current nanopore sequencing methods rely on ionic current measurements from individually addressed pores and such methods are likely to prove difficult to scale up. Here we show that, by optically encoding the ionic flux through protein nanopores, the discrimination of nucleic acid sequences and the detection of sequence-specific nucleic acid hybridization events can be parallelized. We make optical recordings at a density of ∼10(4) nanopores per mm(2) in a single droplet interface bilayer. Nanopore blockades can discriminate between DNAs with sub-picoampere equivalent resolution, and specific miRNA sequences can be identified by differences in unzipping kinetics. By creating an array of 2,500 bilayers with a micropatterned hydrogel chip, we are also able to load different samples into specific bilayers suitable for high-throughput nanopore recording.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.