Evidence map›Paper›PMID 26322837›Full record

ArticleNature methods2015

Inntags: small self-structured epitopes for innocuous protein tagging.

Maya V Georgieva, Galal Yahya, Laia Codó, Raúl Ortiz, Laura Teixidó, José Claros, Ricardo Jara, Mònica Jara, Antoni Iborra, Josep Lluís Gelpí and 3 more

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Article in Nature methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Maya V GeorgievaMolecular Biology Institute of Barcelona (IBMB), Consejo Superior de Investigaciones Científicas (CSIC), Barcelona, Spain.
Galal YahyaMolecular Biology Institute of Barcelona (IBMB), Consejo Superior de Investigaciones Científicas (CSIC), Barcelona, Spain.
Laia CodóBarcelona Supercomputing Center (BSC), Barcelona, Spain.
Raúl OrtizMolecular Biology Institute of Barcelona (IBMB), Consejo Superior de Investigaciones Científicas (CSIC), Barcelona, Spain.
Laura TeixidóAbBcn Ltd, Bellaterra, Spain.
José ClarosImmunostep Ltd, Salamanca, Spain.
Ricardo JaraImmunostep Ltd, Salamanca, Spain.
Mònica JaraAbBcn Ltd, Bellaterra, Spain.
Antoni IborraAbBcn Ltd, Bellaterra, Spain.
Josep Lluís GelpíBarcelona Supercomputing Center (BSC), Barcelona, Spain.ORCID http://orcid.org/0000-0002-0566-7723
Carme GallegoMolecular Biology Institute of Barcelona (IBMB), Consejo Superior de Investigaciones Científicas (CSIC), Barcelona, Spain.
Modesto OrozcoJoint BSC-CRG-IRB Programme in Computational Biology, Barcelona, Spain.
Martí AldeaMolecular Biology Institute of Barcelona (IBMB), Consejo Superior de Investigaciones Científicas (CSIC), Barcelona, Spain.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Protein tagging is widely used in approaches ranging from affinity purification to fluorescence-based detection in live cells. However, an intrinsic limitation of tagging is that the native function of the protein may be compromised or even abolished by the presence of the tag. Here we describe and characterize a set of small, innocuous protein tags (inntags) that we anticipate will find application in a variety of biological techniques.

Indexed as

AnimalsAntibodies, MonoclonalEpitopesFemaleFluorescent Antibody TechniqueGreen Fluorescent ProteinsHEK293 CellsHumansImmunoprecipitationMiceMice, Inbred BALB CNIH 3T3 CellsProtein ConformationProteinsProtein Structure, TertiaryRecombinant ProteinsAntibodies, MonoclonalEpitopesGreen Fluorescent ProteinsProteinsRecombinant Proteins

Identifiers

PMID26322837

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.