Evidence map›Paper›PMID 26322053›Full record

ArticleFrontiers in plant science2015

Production of polyhydroxybutyrate in oil palm (Elaeis guineensis Jacq.) mediated by microprojectile bombardment of PHB biosynthesis genes into embryogenic calli.

Ghulam Kadir Ahmad Parveez, Bohari Bahariah, Nor Hanin Ayub, Mat Yunus Abdul Masani, Omar Abdul Rasid, Ahmad Hashim Tarmizi, Zamzuri Ishak

Open access · goldAbstract read
In one paragraph

Article in Frontiers in plant science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
3.0field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 51 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Ghulam Kadir Ahmad ParveezAdvanced Biotechnology and Breeding Centre, Malaysian Palm Oil Board Kajang, Malaysia.
Bohari BahariahAdvanced Biotechnology and Breeding Centre, Malaysian Palm Oil Board Kajang, Malaysia.
Nor Hanin AyubAdvanced Biotechnology and Breeding Centre, Malaysian Palm Oil Board Kajang, Malaysia.
Mat Yunus Abdul MasaniAdvanced Biotechnology and Breeding Centre, Malaysian Palm Oil Board Kajang, Malaysia.
Omar Abdul RasidAdvanced Biotechnology and Breeding Centre, Malaysian Palm Oil Board Kajang, Malaysia.
Ahmad Hashim TarmiziAdvanced Biotechnology and Breeding Centre, Malaysian Palm Oil Board Kajang, Malaysia.
Zamzuri IshakAdvanced Biotechnology and Breeding Centre, Malaysian Palm Oil Board Kajang, Malaysia.
Malaysian Palm Oil Board · MY

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Biodegradable plastics, mainly polyhydroxybutyrate (PHB), which are traditionally produced by bacterial cells, have been produced in the cells of more than 15 plant species. Since the production of biodegradable plastics and the synthesis of oil in plants share the same substrate, acetyl-coenzyme A (acetyl-CoA), producing PHB in oil bearing crops, such as oil palm, will be advantageous. In this study, three bacterial genes, bktB, phaB, and phaC, which are required for the synthesis of PHB and selectable marker gene, bar, for herbicide Basta resistant, were transformed into embryogenic calli. A number of transformed embryogenic lines resistant to herbicide Basta were obtained and were later regenerated to produce few hundred plantlets. Molecular analyses, including polymerase chain reaction (PCR), Southern blot, and real-time PCR have demonstrated stable integration and expression of the transgenes in the oil palm genome. HPLC and Nile blue A staining analyses confirmed the synthesis of PHB in some of the plantlets.

Indexed as

biodegradable plasticsbiolisticsmonocotoil palmtransgenic

Identifiers

PMID26322053
PMCPMC4531230
OpenAlexW1679469203

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.