ArticleFrontiers in plant science2015
Meristem micropropagation of cassava (Manihot esculenta) evokes genome-wide changes in DNA methylation.
Article in Frontiers in plant science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Understanding and modifying starch metabolism to limit yield losses in field-grown cassava.Plant physiology · 2026Article
- Detection of differential DNA methylation in date palm (Phoenix dactylifera L.) vitroplants using whole genome bisulfite sequencing analysis.BMC plant biology · 2026Article
- Micropropagation of Cannabis sativa: genetic and epigenetic stability assessment over multiple generations.Journal of cannabis research · 2026Article
- Effect of sterilants and plant growth regulators in regenerating commonly used cassava cultivars at the Kenyan coast.Heliyon · 2023Article
- Additive and non-additive epigenetic signatures of natural hybridization between fish species with different mating systems.Epigenetics · 2022Article
- Against the Odds: Hybrid Zones between Mangrove Killifish Species with Different Mating Systems.Genes · 2021Article
- Differential DNA methylation and gene expression during development of reproductive and vegetative organs in Ilex species.Journal of plant research · 2021Article
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- Greenhouse Spatial Effects Detected in the Barley (Frontiers in plant science · 2020Article
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- Agromorphologic, genetic and methylation profiling of Dioscorea and Musa species multiplied under three micropropagation systems.PloS one · 2019Article
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- Multiple morphogenic culture systems cause loss of resistance to cassava mosaic disease.BMC plant biology · 2018Article
- Plant Stress Responses and Phenotypic Plasticity in the Epigenomics Era: Perspectives on the Grapevine Scenario, a Model for Perennial Crop Plants.Frontiers in plant science · 2017Article
- Global DNA Methylation Patterns Can Play a Role in Defining Terroir in Grapevine (Frontiers in plant science · 2017Article
- A Concise Review on Multi-Omics Data Integration for Terroir Analysis inFrontiers in plant science · 2017Review
- Widespread natural variation of DNA methylation within angiosperms.Genome biology · 2016Article
- Article
- Effect of Cryopreservation and Post-Cryopreservation Somatic Embryogenesis on the Epigenetic Fidelity of Cocoa (Theobroma cacao L.).PloS one · 2016Article
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6 authors.
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Abstract
There is great interest in the phenotypic, genetic and epigenetic changes associated with plant in vitro culture known as somaclonal variation. In vitro propagation systems that are based on the use of microcuttings or meristem cultures are considered analogous to clonal cuttings and so widely viewed to be largely free from such somaclonal effects. In this study, we surveyed for epigenetic changes during propagation by meristem culture and by field cuttings in five cassava (Manihot esculenta) cultivars. Principal Co-ordinate Analysis of profiles generated by methylation-sensitive amplified polymorphism revealed clear divergence between samples taken from field-grown cuttings and those recovered from meristem culture. There was also good separation between the tissues of field samples but this effect was less distinct among the meristem culture materials. Application of methylation-sensitive Genotype by sequencing identified 105 candidate epimarks that distinguish between field cutting and meristem culture samples. Cross referencing the sequences of these epimarks to the draft cassava genome revealed 102 sites associated with genes whose homologs have been implicated in a range of fundamental biological processes including cell differentiation, development, sugar metabolism, DNA methylation, stress response, photosynthesis, and transposon activation. We explore the relevance of these findings for the selection of micropropagation systems for use on this and other crops.
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