ArticleMolecular vision2015
Distinctive and pervasive alterations in aqueous humor protein composition following different types of glaucoma surgery.
Article in Molecular vision, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 43 citations in OpenAlex.
- Aqueous Humor Total Protein Levels Predict 6-Year Graft Survival after Endothelial Keratoplasty in Surgically Treated Glaucoma Eyes.Ophthalmology science · 2026Article
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- Descemet Membrane Endothelial Keratoplasty for Penetrating Keratoplasty.Journal of pharmacy & bioallied sciences · 2024Article
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- Effect of guided Ahmed glaucoma valve implantation on corneal endothelial cells: A 2-year comparative study.PloS one · 2023Article
- Etiology and outcome of penetrating keratoplasty in bullous keratopathy post-cataract surgery vs post-glaucoma surgery.PloS one · 2023Article
- Outcomes of Ahmed glaucoma valve implantation and risk factors for graft failure in eyes with Descemet's stripping endothelial keratoplasty.International ophthalmology · 2022Article
- Current Perspectives on Corneal Transplantation.Clinical ophthalmology (Auckland, N.Z.) · 2022Review
- Evolving Techniques and Indications of Descemet Membrane Endothelial Keratoplasty.Turkish journal of ophthalmology · 2021Review
- Aqueous humour cytokine profiles after Descemet's membrane endothelial keratoplasty.Scientific reports · 2021Article
- The Effect of Tube Location on Corneal Endothelial Cells in Patients with Ahmed Glaucoma Valve.Ophthalmology · 2021Article
- Study of minimally invasive radiofrequency ablation of the ciliary body for the treatment of glaucoma in rabbits.Molecular medicine reports · 2020Article
- Five-year follow-up outcomes after Descemet's stripping automated endothelial keratoplasty: a retrospective study.BMJ open ophthalmology · 2020Article
- Aqueous humor protein dysregulation in primary angle-closure glaucoma.International ophthalmology · 2019Article
- The Human Eye Proteome Project: Updates on an Emerging Proteome.Proteomics · 2018Review
- Anterior Chamber Flare as an Objective and Quantitative Noninvasive Method for Oculopathy in Transthyretin V30M Amyloidosis Patients.Journal of ophthalmology · 2018Article
- Personalized Medicine in Ocular Fibrosis: Myth or Future Biomarkers.Advances in wound care · 2016Review
- Challenges in the complex management of post-keratoplasty glaucoma.Therapeutic advances in ophthalmologyReview
- Likelihood of Corneal Transplantation after Tube versus Trabeculectomy: An IRIS® Registry Study.Ophthalmology. GlaucomaArticle
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo investigate whether specific glaucoma surgeries are associated with differences in aqueous humor protein concentrations compared to eyes without filters.
methodsIn this cross-sectional study, aqueous humor samples were prospectively collected from control subjects who underwent routine cataract surgery (n=14) and from patients who had different glaucoma filters: Baerveldt aqueous shunt (n=6), Ahmed aqueous shunt (n=6), trabeculectomy (n=5), and Ex-Press trabeculectomy (n=3). Total protein concentrations were determined with Bradford assay. Tryptic digests were analyzed with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Proteins were identified with high confidence using stringent criteria and were quantitatively compared with a label-free platform. Relative protein quantities were compared across groups with ANOVA. Post hoc pair-wise comparisons were adjusted for multiple comparisons.
resultsCompared to the control eyes, the aqueous humor protein concentration was increased approximately tenfold in the Ahmed and Baerveldt eyes and fivefold in the trabeculectomy and Ex-Press eyes. Overall, 718 unique proteins, splice variants, or isoforms were identified. No differences in the protein concentrations were detected between the Baerveldt and Ahmed groups. Likewise, the trabeculectomy and Ex-Press groups were remarkably similar. Therefore, the aqueous shunt groups were pooled, and the trabeculectomy groups were pooled for a three-way comparison with the controls. More than 500 proteins differed significantly in relative abundance (ANOVA p<0.01) among the control, aqueous shunt, and trabeculectomy groups. Functional analyses suggested these alterations in relative protein abundance affected dozens of signaling pathways.
conclusionsDifferent glaucoma surgical procedures were associated with marked increases in the aqueous humor protein concentration and distinctive changes in the relative abundance of numerous proteins involved in multiple signaling pathways.
Indexed as
Identifiers
26321865PMC4548793W1861332739What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.