Evidence map›Paper›PMID 26321278›Full record

ReviewPharmacological reports : PR2015

Toward selective detection of reactive oxygen and nitrogen species with the use of fluorogenic probes--Limitations, progress, and perspectives.

Karolina Debowska, Dawid Debski, Micael Hardy, Malgorzata Jakubowska, Balaraman Kalyanaraman, Andrzej Marcinek, Radosław Michalski, Bartosz Michalowski, Olivier Ouari, Adam Sikora and 2 more

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Pharmacological reports : PR, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 1 synthesis or guideline pooled it.

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  17. Fluorescence quenching by metal centered porphyrins and poryphyrin enzymes.American journal of physiology. Regulatory, integrative and comparative physiology · 2017
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Karolina DebowskaInstitute of Applied Radiation Chemistry, Lodz University of Technology, Łódź, Poland.
Dawid DebskiInstitute of Applied Radiation Chemistry, Lodz University of Technology, Łódź, Poland.
Micael HardyAix-Marseille Université, CNRS, ICR UMR 7273, SREP, Centre de Saint Jérôme, Marseille Cedex 20, France.
Malgorzata JakubowskaInstitute of Applied Radiation Chemistry, Lodz University of Technology, Łódź, Poland.
Balaraman KalyanaramanDepartment of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, USA.
Andrzej MarcinekInstitute of Applied Radiation Chemistry, Lodz University of Technology, Łódź, Poland.
Radosław MichalskiInstitute of Applied Radiation Chemistry, Lodz University of Technology, Łódź, Poland.
Bartosz MichalowskiInstitute of Applied Radiation Chemistry, Lodz University of Technology, Łódź, Poland.
Olivier OuariAix-Marseille Université, CNRS, ICR UMR 7273, SREP, Centre de Saint Jérôme, Marseille Cedex 20, France.
Adam SikoraInstitute of Applied Radiation Chemistry, Lodz University of Technology, Łódź, Poland. Electronic address: adam.sikora@p.lodz.pl.
Renata SmulikInstitute of Applied Radiation Chemistry, Lodz University of Technology, Łódź, Poland.
Jacek ZielonkaDepartment of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, USA.

Funding

CYP2E1 Mediated Mitochondrial Injury and Cell Damage in Alcohol Liver DiseaseR01AA022986 · NIAAA · UNIVERSITY OF PENNSYLVANIA · PI AVADHANI, NARAYAN G, KALYANARAMAN, BALARAMAN · 2015 to 2019
$2.6M
Targeting pancreatic cancer energy metabolism, tumor growth, and metastasisU01CA178960 · NCI · MEDICAL COLLEGE OF WISCONSIN · PI DWINELL, MICHAEL B, KALYANARAMAN, BALARAMAN · 2014 to 2018
$2.6M
NCI NIH HHS U01 CA178960NIAAA NIH HHS R01 AA022986
6 · The paper itself

Abstract

Over the last 40 years, there has been tremendous progress in understanding the biological reactions of reactive oxygen species (ROS) and reactive nitrogen species (RNS). It is widely accepted that the generation of ROS and RNS is involved in physiological and pathophysiological processes. To understand the role of ROS and RNS in a variety of pathologies, the specific detection of ROS and RNS is fundamental. Unfortunately, the intracellular detection and quantitation of ROS and RNS remains a challenge. In this short review, we have focused on the mechanistic and quantitative aspects of their detection with the use of selected fluorogenic probes. The challenges, limitations and perspectives of these methods are discussed.

Indexed as

AnimalsFluorescent DyesHumansReactive Nitrogen SpeciesReactive Oxygen SpeciesFluorescent DyesReactive Nitrogen SpeciesReactive Oxygen SpeciesFluorogenic probesHydrogen peroxidePeroxynitriteProtein hydroperoxidesSuperoxide radical anion

Identifiers

PMID26321278
PMCPMC5793900

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.