ArticlePlant physiology2015
The RING E3 Ligase KEEP ON GOING Modulates JASMONATE ZIM-DOMAIN12 Stability.
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Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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43 citing papers in PubMed, 93 citations in OpenAlex.
- The role of jasmonates in plant root growth and development.Frontiers in plant science · 2026Review
- Molecular Decoding of Phytohormone Crosstalk: JA-Mediated Key Regulatory Nodes and Signal Integration.Plants (Basel, Switzerland) · 2025Review
- Review
- Genome-wide identification of F-box-LRR gene family and the functional analysis of CsFBXL13 transcription factor in tea plants.Functional & integrative genomics · 2025Article
- Hypoxia represses pattern-triggered immune responses in Arabidopsis.Plant physiology · 2024Article
- Crosstalk between Ethylene, Jasmonate and ABA in Response to Salt Stress during Germination and Early Plant Growth inInternational journal of molecular sciences · 2024Article
- The RING-Type E3 Ubiquitin Ligase GenePlants (Basel, Switzerland) · 2024Article
- Arabidopsis HECT and RING-type E3 Ligases Promote MAPKKK18 Degradation to Regulate Abscisic Acid Signaling.Plant & cell physiology · 2024Article
- The Arabidopsis F-box protein SKP1-INTERACTING PARTNER 31 modulates seed maturation and seed vigor by targeting JASMONATE ZIM DOMAIN proteins independently of jasmonic acid-isoleucine.The Plant cell · 2023Article
- Jasmonate-regulated root growth inhibition and root hair elongation.Journal of experimental botany · 2023Review
- Emerging roles of the ubiquitin-proteasome pathway in enhancing crop yield by optimizing seed agronomic traits.Plant cell reports · 2022Review
- ABI5 binding protein2 inhibits ABA responses during germination without ABA-INSENSITIVE5 degradation.Plant physiology · 2022Article
- Identification of TIFY gene family in walnut and analysis of its expression under abiotic stresses.BMC genomics · 2022Article
- Gene Expression Analysis of Potato (Plants (Basel, Switzerland) · 2022Article
- Interactome of Arabidopsis Thaliana.Plants (Basel, Switzerland) · 2022Article
- Functional pleiotropism, diversity, and redundancy ofHorticulture research · 2022Article
- Identification and expression analysis ofPlant signaling & behavior · 2021Article
- Factors that affect protein abundance of a positive regulator of abscisic acid signalling, the basic leucine zipper transcription factor ABRE-binding factor 2 (ABF2).Plant direct · 2021Article
- Jasmonates and Plant Salt Stress: Molecular Players, Physiological Effects, and Improving Tolerance by Using Genome-Associated Tools.International journal of molecular sciences · 2021Review
- Jasmonic Acid Signaling and Molecular Crosstalk with Other Phytohormones.International journal of molecular sciences · 2021Review
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Authors and funding
18 authors at 4 institutions in 4 countries.
Laurens PauwelsDepartment of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).ORCID http://orcid.org/0000-0002-0221-9052
Andrés RitterDepartment of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).
Jonas GoossensDepartment of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).
Astrid Nagels DurandDepartment of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).
Hongxia LiuDepartment of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).ORCID http://orcid.org/0000-0002-9232-5122
Yangnan GuDepartment of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).ORCID http://orcid.org/0000-0001-5135-0180
Jan GeerinckDepartment of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).ORCID http://orcid.org/0000-0002-1003-1228
Marta BoterDepartment of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).ORCID http://orcid.org/0000-0001-8688-5513
Robin Vanden BosscheDepartment of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).ORCID http://orcid.org/0000-0002-4593-9658
Rebecca De ClercqDepartment of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).ORCID http://orcid.org/0000-0002-4068-1811
Jelle Van LeeneDepartment of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).ORCID http://orcid.org/0000-0002-4932-8192
Kris GevaertDepartment of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).ORCID http://orcid.org/0000-0002-4237-0283
Geert De JaegerDepartment of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).ORCID http://orcid.org/0000-0001-6558-5669
Roberto SolanoDepartment of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).
Sophia StoneDepartment of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).
Roger W InnesDepartment of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).
Judy CallisDepartment of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.).ORCID http://orcid.org/0000-0002-0622-078X
Alain GoossensDepartment of Plant Systems Biology, Flanders Institute for Biotechnology, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (L.P., A.R., J.Go., A.N.D., J.Ge., R.V.B., R.D.C., J.V.L., G.D.J., A.G.);Department of Molecular and Cellular Biology, University of California, Davis, California 95616 (L.P., J.C.);Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4R2 (H.L., S.S.);Department of Biology, Indiana University, Bloomington, Indiana 47405 (Y.Gu, R.W.I.);Plant Molecular Genetics Department, National Centre for Biotechnology, Consejo Superior de Investigaciones Científicas, Campus University Autónoma, 28049 Madrid, Spain (M.B., R.S.);Department of Medical Protein Research, Flanders Institute for Biotechnology, 9000 Ghent, Belgium (K.G.); andDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium (K.G.) algoo@psb.vib-ugent.be.ORCID http://orcid.org/0000-0002-1599-551X
Vlaams Instituut voor Biotechnologie · BEVIB-UGent Center for Plant Systems Biology · BEConsejo Superior de Investigaciones Científicas · ESDalhousie University · CA
Funding
Programmed Cell Death and Disease Resistance in ArabidopsisR01GM063761 · NIGMS · TRUSTEES OF INDIANA UNIVERSITY · PI INNES, ROGER W. · 2002 to 2013
$3.5MNIGMS NIH HHS R01 GM063761
Abstract
Jasmonate (JA) signaling in plants is mediated by the JASMONATE ZIM-DOMAIN (JAZ) proteins that repress the activity of several transcription factors regulating JA-inducible gene expression. The hormone JA-isoleucine triggers the interaction of JAZ repressor proteins with the F-box protein CORONATINE INSENSITIVE1 (COI1), part of an S-phase kinase-associated protein1/Cullin1/F-box protein COI1 (SCF(COI1)) E3 ubiquitin ligase complex, and their degradation by the 26S proteasome. In Arabidopsis (Arabidopsis thaliana), the JAZ family consists of 13 members. The level of redundancy or specificity among these members is currently not well understood. Here, we characterized JAZ12, encoded by a highly expressed JAZ gene. JAZ12 interacted with the transcription factors MYC2, MYC3, and MYC4 in vivo and repressed MYC2 activity. Using tandem affinity purification, we found JAZ12 to interact with SCF(COI1) components, matching with observed in vivo ubiquitination and with rapid degradation after treatment with JA. In contrast to the other JAZ proteins, JAZ12 also interacted directly with the E3 RING ligase KEEP ON GOING (KEG), a known repressor of the ABSCISIC ACID INSENSITIVE5 transcription factor in abscisic acid signaling. To study the functional role of this interaction, we circumvented the lethality of keg loss-of-function mutants by silencing KEG using an artificial microRNA approach. Abscisic acid treatment promoted JAZ12 degradation, and KEG knockdown led to a decrease in JAZ12 protein levels. Correspondingly, KEG overexpression was capable of partially inhibiting COI1-mediated JAZ12 degradation. Our results provide additional evidence for KEG as an important factor in plant hormone signaling and a positive regulator of JAZ12 stability.
Indexed as
Abscisic AcidArabidopsisArabidopsis ProteinsBasic Helix-Loop-Helix Leucine Zipper Transcription FactorsGene Expression Regulation, PlantGene Knockdown TechniquesMutationNicotianaPlants, Genetically ModifiedProtein StabilityProtein Structure, TertiaryRepressor ProteinsUbiquitin-Protein LigasesAbscisic AcidArabidopsis ProteinsBasic Helix-Loop-Helix Leucine Zipper Transcription FactorsCOI1 protein, ArabidopsisJAZ12 protein, ArabidopsisKEG protein, ArabidopsisMYC2 protein, ArabidopsisRepressor ProteinsUbiquitin-Protein Ligases
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