ArticleMicrobial pathogenesis2016
Sialic acid transporter NanT participates in Tannerella forsythia biofilm formation and survival on epithelial cells.
Article in Microbial pathogenesis, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 21 citations in OpenAlex.
- Review
- Obtaining Muramic Acid fromLife (Basel, Switzerland) · 2025Article
- The intriguing strategies ofFrontiers in oral health · 2024Article
- Structural and functional characterisation of a stable, broad-specificity multimeric sialidase from the oral pathogen Tannerella forsythia.The Biochemical journal · 2022Article
- Production of nonulosonic acids in the extracellular polymeric substances of "Candidatus Accumulibacter phosphatis".Applied microbiology and biotechnology · 2021Article
- Comparative genome characterization of the periodontal pathogen Tannerella forsythia.BMC genomics · 2020Article
- [Research progress on carbohydrate active enzymes of human microbiome].Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology · 2019Review
- Sialidase and N-acetylneuraminate catabolism in nutrition of Mycoplasma alligatoris.Microbiology (Reading, England) · 2019Article
- Peptidoglycan synthesis in Tannerella forsythia: Scavenging is the modus operandi.Molecular oral microbiology · 2018Review
- Mycobacterium tuberculosis Major Facilitator Superfamily Transporters.The Journal of membrane biology · 2017Review
- Structure of the LPS O-chain from Fusobacterium nucleatum strain 10953, containing sialic acid.Carbohydrate research · 2017Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Tannerella forsythia is a periodontal pathogen implicated in periodontitis. This gram-negative pathogen depends on exogenous peptidoglycan amino sugar N-acetylmuramic acid (NAM) for growth. In the biofilm state the bacterium can utilize sialic acid (Neu5Ac) instead of NAM to sustain its growth. Thus, the sialic acid utilization system of the bacterium plays a critical role in the growth and survival of the organism in the absence of NAM. We sought the function of a T. forsythia gene annotated as nanT coding for an inner-membrane sugar transporter located on a sialic acid utilization genetic cluster. To determine the function of this putative sialic acid transporter, an isogenic nanT-deletion mutant generated by allelic replacement strategy was evaluated for biofilm formation on NAM or Neu5Ac, and survival on KB epithelial cells. Moreover, since T. forsythia forms synergistic biofilms with Fusobacterium nucleatum, co-biofilm formation activity in mixed culture and sialic acid uptake in culture were also assessed. The data showed that the nanT-inactivated mutant of T. forsythia was attenuated in its ability to uptake sialic acid. The mutant formed weaker biofilms compared to the wild-type strain in the presence of sialic acid and as co-biofilms with F. nucleatum. Moreover, compared to the wild-type T. forsythia nanT-inactivated mutant showed reduced survival when incubated on KB epithelial cells. Taken together, the data presented here demonstrate that NanT-mediated sialic transportation is essential for sialic acid utilization during biofilm growth and survival of the organism on epithelial cells and implies sialic acid might be key for its survival both in subgingival biofilms and during infection of human epithelial cells in vivo.
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