ArticleMolecular biology and evolution2015
Extensive Families of miRNAs and PHAS Loci in Norway Spruce Demonstrate the Origins of Complex phasiRNA Networks in Seed Plants.
Article in Molecular biology and evolution, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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Who cites it
75 citing papers in PubMed, 148 citations in OpenAlex.
- Small RNA mobility and plant virus diseases.Journal of experimental botany · 2026Review
- Dual RNA-seq analysis reveals differences in defensive lncRNA expression in Pinus spp. with varying susceptibility to Fusarium circinatum.BMC plant biology · 2026Article
- Epigenetics in the modern era of crop improvements.Science China. Life sciences · 2025Review
- Unraveling the Multilayered Regulatory Networks of miRNAs and PhasiRNAs inPlants (Basel, Switzerland) · 2025Article
- Sexual reproduction in land plants: an evolutionary perspective.Plant reproduction · 2025Review
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- The role of microRNAs in NBS-LRR gene expression and its implications for plant immunity and crop development.Transgenic research · 2024Review
- Epigenetic stress memory in gymnosperms.Plant physiology · 2024Review
- Comparative analysis, diversification, and functional validation of plant nucleotide-binding site domain genes.Scientific reports · 2024Article
- MicroRNA482/2118 is lineage-specifically involved in gibberellin signalling via the regulation of GID1 expression by targeting noncoding PHAS genes and subsequently instigated phasiRNAs.Plant biotechnology journal · 2024Article
- Phased secondary small interfering RNAs inNAR genomics and bioinformatics · 2023Article
- Regulation of plant immunity via small RNA-mediated control of NLR expression.Journal of experimental botany · 2023Article
- The genome of the Wollemi pine, a critically endangered "living fossil" unchanged since the Cretaceous, reveals extensive ancient transposon activity.bioRxiv : the preprint server for biology · 2023Article
- CottonBiology · 2023Article
- Miniature Inverted-Repeat Transposable Elements: Small DNA Transposons That Have Contributed to PlantPlants (Basel, Switzerland) · 2023Review
- Article
- Biogenesis, conservation, and function of miRNA in liverworts.Journal of experimental botany · 2022Review
- The evolutionary history of small RNAs in Solanaceae.Plant physiology · 2022Article
- Role of phasiRNAs in plant-pathogen interactions: molecular perspectives and bioinformatics tools.Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Review
- The Multiverse of Plant Small RNAs: How Can We Explore It?International journal of molecular sciences · 2022Review
15 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In eudicot plants, the miR482/miR2118 superfamily regulates and instigates the production of phased secondary small interfering RNAs (siRNAs) from NB-LRR (nucleotide binding leucine-rich repeat) genes that encode disease resistance proteins. In grasses, this miRNA family triggers siRNA production specifically in reproductive tissues from long noncoding RNAs. To understand this functional divergence, we examined the small RNA population in the ancient gymnosperm Norway spruce (Picea abies). As many as 41 miRNA families in spruce were found to trigger phasiRNA (phased, secondary siRNAs) production from diverse PHAS loci, with a remarkable 19 miRNA families capable of targeting over 750 NB-LRR genes to generate phasiRNAs. miR482/miR2118, encoded in spruce by at least 24 precursor loci, targets not only NB-LRR genes to trigger phasiRNA production (as in eudicots) but also noncoding PHAS loci, generating phasiRNAs preferentially in male or female cones, reminiscent of its role in the grasses. These data suggest a dual function of miR482/miR2118 present in gymnosperms that was selectively yet divergently retained in flowering plants. A few MIR482/MIR2118 precursors possess an extremely long stem-loop structure, one arm of which shows significant sequence similarity to spruce NB-LRR genes, suggestive of an evolutionary origin from NB-LRR genes through gene duplication. We also characterized an expanded miR390-TAS3 (TRANS-ACTING SIRNA GENE 3)-ARF (AUXIN RESPONSIVE FACTOR) pathway, comprising 18 TAS3 genes of diverse features. Finally, we annotated spruce miRNAs and their targets. Taken together, these data expand our understanding of phasiRNA network in plants and the evolution of plant miRNAs, particularly miR482/miR2118 and its functional diversification.
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