ArticlePloS one2015
An Engineered Split Intein for Photoactivated Protein Trans-Splicing.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 50 citations in OpenAlex.
- Photoswitchable intein for light control of covalent protein binding and cleavage.Nature communications · 2025Article
- Autonomous Nucleic Acid and Protein Nanocomputing Agents Engineered to Operate in Living Cells.ACS nano · 2025Review
- Conditional Split Inteins: Adaptable Tools for Programming Protein Functions.International journal of molecular sciences · 2025Review
- Distinct phases of cellular signaling revealed by time-resolved protein synthesis.Nature chemical biology · 2024Article
- Int&in: A machine learning-based web server for active split site identification in inteins.Protein science : a publication of the Protein Society · 2024Article
- Irreversible light-activated SpyLigation mediates split-protein assembly in 4D.Nature protocols · 2024Review
- Near-Infrared Optogenetic Module for Conditional Protein Splicing.Journal of molecular biology · 2023Article
- Spatiotemporal functional assembly of split protein pairs through a light-activated SpyLigation.Nature chemistry · 2023Article
- Nature-inspired protein ligation and its applications.Nature reviews. Chemistry · 2023Review
- Optogenetics in bacteria - applications and opportunities.FEMS microbiology reviews · 2022Review
- A light way for nuclear cell biologists.Journal of biochemistry · 2021Review
- Engineering an Allosteric Control of Protein Function.The journal of physical chemistry. B · 2021Review
- Photo-SNAP-tag, a Light-Regulated Chemical Labeling System.ACS chemical biology · 2020Article
- Light directed migration of a cluster of cells in the centimeter scale.Small GTPases · 2020Article
- Proximity Induced Splicing Utilizing Caged Split Inteins.Journal of the American Chemical Society · 2019Article
- Structure of an engineered intein reveals thiazoline ring and provides mechanistic insight.Biotechnology and bioengineering · 2019Article
- Engineering Allostery into Proteins.Advances in experimental medicine and biology · 2019Review
- Protease-Activatable Scaffold Proteins as Versatile Molecular Hubs in Synthetic Signaling Networks.ACS synthetic biology · 2018Article
- Inteins: Localized Distribution, Gene Regulation, and Protein Engineering for Biological Applications.Microorganisms · 2018Review
- Intein Zymogens: Conditional Assembly and Splicing of Split Inteins via Targeted Proteolysis.Journal of the American Chemical Society · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein splicing is mediated by inteins that auto-catalytically join two separated protein fragments with a peptide bond. Here we engineered a genetically encoded synthetic photoactivatable intein (named LOVInC), by using the light-sensitive LOV2 domain from Avena sativa as a switch to modulate the splicing activity of the split DnaE intein from Nostoc punctiforme. Periodic blue light illumination of LOVInC induced protein splicing activity in mammalian cells. To demonstrate the broad applicability of LOVInC, synthetic protein systems were engineered for the light-induced reassembly of several target proteins such as fluorescent protein markers, a dominant positive mutant of RhoA, caspase-7, and the genetically encoded Ca2+ indicator GCaMP2. Spatial precision of LOVInC was demonstrated by targeting activity to specific mammalian cells. Thus, LOVInC can serve as a general platform for engineering light-based control for modulating the activity of many different proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.