ArticlePloS one2015
Identification of Novel Short Ragweed Pollen Allergens Using Combined Transcriptomic and Immunoproteomic Approaches.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 37 citations in OpenAlex.
- Amb a 1-Specific IgE in Heart Failure: A Translational Framework for Seasonal Risk, Endotyping, and Patient-Centered Management.Journal of clinical medicine · 2026Review
- Expanding grass pollen allergomes: benefits for the molecular profiling of products used forFrontiers in allergy · 2026Article
- The allergenic potential of enolases: physiological and pathophysiological insights.Current opinion in allergy and clinical immunology · 2025Review
- Heterogenous Induction of Blocking Antibodies against Ragweed Allergen Molecules by Allergen Extract-Based Immunotherapy Vaccines.Vaccines · 2024Article
- The ancestral karyotype of the Heliantheae Alliance, herbicide resistance, and human allergens: Insights from the genomes of common and giant ragweed.The plant genome · 2024Article
- Perspectives of Proteomics in Respiratory Allergic Diseases.International journal of molecular sciences · 2023Review
- Molecular allergy diagnosis is sensitive and avoids misdiagnosis in patients sensitized to seasonal allergens.Clinical and translational allergy · 2023Article
- Ragweed Major Allergen Amb a 11 Recombinant Production and Clinical Implications.Biomolecules · 2023Article
- Mechanism and clinical evidence of immunotherapy in allergic rhinitis.Frontiers in allergy · 2023Review
- The Identification and Characterization of Immunoreactive Fungal Proteins Recognized by Sera from Zimbabweans Sensitized to Fungi.International archives of allergy and immunology · 2022Article
- Mechanisms of allergen-specific immunotherapy for allergic rhinitis and food allergies.Bioscience reports · 2020Review
- Article
- Analysis of Pollen Allergens in Lily by Transcriptome and Proteome Data.International journal of molecular sciences · 2019Article
- Ambrosia artemisiifolia L. temperature-responsive traits influencing the prevalence and severity of pollinosis: a study in controlled conditions.BMC plant biology · 2019Article
- Article
- Article
- Proteomic profiling of the weed feverfew, a neglected pollen allergen source.Scientific reports · 2017Article
- Comparative protein profiles of the Ambrosia plants.Biochimica et biophysica acta. Proteins and proteomics · 2017Article
- A combined transcriptome and proteome analysis extends the allergome of house dust mite Dermatophagoides species.PloS one · 2017Article
- Proteomics for Allergy: from Proteins to the Patients.Current allergy and asthma reports · 2016Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAllergy to short ragweed (Ambrosia artemisiifolia) pollen is a serious and expanding health problem in North America and Europe. Whereas only 10 short ragweed pollen allergens are officially recorded, patterns of IgE reactivity observed in ragweed allergic patients suggest that other allergens contribute to allergenicity. The objective of the present study was to identify novel allergens following extensive characterization of the transcriptome and proteome of short ragweed pollen.
methodsFollowing a Proteomics-Informed-by-Transcriptomics approach, a comprehensive transcriptomic data set was built up from RNA-seq analysis of short ragweed pollen. Mass spectrometry-based proteomic analyses and IgE reactivity profiling after high resolution 2D-gel electrophoresis were then combined to identify novel allergens.
resultsShort ragweed pollen transcripts were assembled after deep RNA sequencing and used to inform proteomic analyses, thus leading to the identification of 573 proteins in the short ragweed pollen. Patterns of IgE reactivity of individual sera from 22 allergic patients were assessed using an aqueous short ragweed pollen extract resolved over 2D-gels. Combined with information derived from the annotated pollen proteome, those analyses revealed the presence of multiple unreported IgE reactive proteins, including new Amb a 1 and Amb a 3 isoallergens as well as 7 novel candidate allergens reacting with IgEs from 20-70% of patients. The latter encompass members of the carbonic anhydrase, enolase, galactose oxidase, GDP dissociation inhibitor, pathogenesis related-17, polygalacturonase and UDP-glucose pyrophosphorylase families.
conclusionsWe extended the list of allergens identified in short ragweed pollen. These findings have implications for both diagnosis and allergen immunotherapy purposes.
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