ArticleBiotechnology progress
Morphological and functional characterization of human induced pluripotent stem cell-derived neurons (iCell Neurons) in defined culture systems.
Article in Biotechnology progress. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 25 citations in OpenAlex.
- Long-Term Engraftment of Cryopreserved Human Neurons for In Vivo Disease Modeling in Neurodegenerative Disease.Biology · 2025Article
- Validation of a functional human AD model with four AD therapeutics utilizing patterned ipsc-derived cortical neurons integrated with microelectrode arrays.Scientific reports · 2024Article
- Validation of a functional human AD model with four AD therapeutics utilizing patterned iPSC-derived cortical neurons integrated with microelectrode arrays.Research square · 2024Article
- Passage number affects differentiation of sensory neurons from human induced pluripotent stem cells.Scientific reports · 2022Article
- Adventures and Advances in Time Travel With Induced Pluripotent Stem Cells and Automated Patch Clamp.Frontiers in molecular neuroscience · 2022Review
- Article
- A human induced pluripotent stem cell-derived cortical neuron human-on-a chip system to study AβAlzheimer's & dementia (New York, N. Y.) · 2020Article
- An Epilepsy-Associated KCNT1 Mutation Enhances Excitability of Human iPSC-Derived Neurons by Increasing Slack KThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Characterization of Functional Primary Cilia in Human Induced Pluripotent Stem Cell-Derived Neurons.Neurochemical research · 2019Article
- Temporal Characterization of Neuronal Migration Behavior on Chemically Patterned Neuronal Circuits in a DefinedACS biomaterials science & engineering · 2018Article
- Physiological Aβ Concentrations Produce a More Biomimetic Representation of the Alzheimer's Disease Phenotype in iPSC Derived Human Neurons.ACS chemical neuroscience · 2018Article
- High-throughput screen for compounds that modulate neurite growth of human induced pluripotent stem cell-derived neurons.Disease models & mechanisms · 2018Article
- Astrocyte-enriched feeder layers from cryopreserved cells support differentiation of spontaneously active networks of human iPSC-derived neurons.Journal of neuroscience methods · 2018Article
- A functional human motor unit platform engineered from human embryonic stem cells and immortalized skeletal myoblasts.Stem cells and cloning : advances and applications · 2018Article
- Multiorgan Microphysiological Systems for Drug Development: Strategies, Advances, and Challenges.Advanced healthcare materials · 2018Review
- Self-contained, low-cost Body-on-a-Chip systems for drug development.Experimental biology and medicine (Maywood, N.J.) · 2017Review
- Altered neurite morphology and cholinergic function of induced pluripotent stem cell-derived neurons from a patient with Kleefstra syndrome and autism.Translational psychiatry · 2017Article
- Article
- Stretch Injury of Human Induced Pluripotent Stem Cell Derived Neurons in a 96 Well Format.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Pre-clinical testing of drug candidates in animal models is expensive, time-consuming, and often fails to predict drug effects in humans. Industry and academia alike are working to build human-based in vitro test beds and advanced high throughput screening systems to improve the translation of preclinical results to human drug trials. Human neurons derived from induced pluripotent stems cells (hiPSCs) are readily available for use within these test-beds and high throughput screens, but there remains a need to robustly evaluate cellular behavior prior to their incorporation in such systems. This study reports on the characterization of one source of commercially available hiPSC-derived neurons, iCell(®) Neurons, for their long-term viability and functional performance to assess their suitability for integration within advanced in vitro platforms. The purity, morphology, survival, identity, and functional maturation of the cells utilizing different culture substrates and medium combinations were evaluated over 28 days in vitro (DIV). Patch-clamp electrophysiological data demonstrated increased capacity for repetitive firing of action potentials across all culture conditions. Significant differences in cellular maturity, morphology, and functional performance were observed in the different conditions, highlighting the importance of evaluating different surface types and growth medium compositions for application in specific in vitro protocols.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.