ArticlePloS one2015
Characterization of Peptidyl-Prolyl Cis-Trans Isomerase- and Calmodulin-Binding Activity of a Cytosolic Arabidopsis thaliana Cyclophilin AtCyp19-3.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- Proteomic analysis of rose apple reveals defense proteins activated during Lasiodiplodia theobromae infection.International microbiology : the official journal of the Spanish Society for Microbiology · 2026Article
- Structural and Kinetic Variability of Pathogen Cyclophilins: Functional Diversification and Therapeutic Potential.ACS infectious diseases · 2026Review
- Construction of Bovine CypA Gene Expression Vector and Validation of Its Expression in CHO-K1 Cells.Animals : an open access journal from MDPI · 2026Article
- Novel resources to investigate leaf plasmodesmata formation in CThe Plant journal : for cell and molecular biology · 2024Article
- Genome-wide identification and expression analyses ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- Characterization and regulation of salt upregulated cyclophilin from a halotolerant strain of Penicillium oxalicum.Scientific reports · 2023Article
- Goss's Wilt Resistance in Corn Is Mediated via Salicylic Acid and Programmed Cell Death but Not Jasmonic Acid Pathways.Plants (Basel, Switzerland) · 2023Article
- Paired growth of cultivated and halophytic wild rice under salt stress induces bacterial endophytes and gene expression responses.Frontiers in plant science · 2023Article
- Novel molecules and target genes for vegetative heat tolerance in wheat.Plant-environment interactions (Hoboken, N.J.) · 2022Article
- Multi-Approach Analysis Reveals Pathways of Cold Tolerance Divergence inFrontiers in plant science · 2022Article
- Molecular Programming of Drought-ChallengedFrontiers in microbiology · 2021Article
- Molecular characterization of a fungal cyclophilin allergen Rhi o 2 and elucidation of antigenic determinants responsible for IgE-cross-reactivity.The Journal of biological chemistry · 2020Article
- Plant Cyclophilins: Multifaceted Proteins With Versatile Roles.Frontiers in plant science · 2020Review
- Comparative Proteomic and Morpho-Physiological Analyses of Maize Wild-Type Vp16 and Mutant vp16 Germinating Seed Responses to PEG-Induced Drought Stress.International journal of molecular sciences · 2019Article
- Enzyme activity and structural features of three single-domain phloem cyclophilins from Brassica napus.Scientific reports · 2019Article
- Gene Expression analysis associated with salt stress in a reciprocally crossed rice population.Scientific reports · 2019Article
- Functional Analysis of Peptidyl-prolylInternational journal of molecular sciences · 2019Article
- The rolB plant oncogene affects multiple signaling protein modules related to hormone signaling and plant defense.Scientific reports · 2018Article
- Bioinformatic and expression analysis of the Brassica napus L. cyclophilins.Scientific reports · 2017Article
- Function of Cyclophilin1 as a long-distance signal molecule in the phloem of tomato plants.Journal of experimental botany · 2017Article
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Authors and funding
11 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cyclophilins, which bind to immunosuppressant cyclosporin A (CsA), are ubiquitous proteins and constitute a multigene family in higher organisms. Several members of this family are reported to catalyze cis-trans isomerisation of the peptidyl-prolyl bond, which is a rate limiting step in protein folding. The physiological role of these proteins in plants, with few exceptions, is still a matter of speculation. Although Arabidopsis genome is predicted to contain 35 cyclophilin genes, biochemical characterization, imperative for understanding their cellular function(s), has been carried only for few of the members. The present study reports the biochemical characterization of an Arabidopsis cyclophilin, AtCyp19-3, which demonstrated that this protein is enzymatically active and possesses peptidyl-prolyl cis-trans isomerase (PPIase) activity that is specifically inhibited by CsA with an inhibition constant (Ki) of 18.75 nM. The PPIase activity of AtCyp19-3 was also sensitive to Cu(2+), which covalently reacts with the sulfhydryl groups, implying redox regulation. Further, using calmodulin (CaM) gel overlay assays it was demonstrated that in vitro interaction of AtCyp19-3 with CaM is Ca(2+)-dependent, and CaM-binding domain is localized to 35-70 amino acid residues in the N-terminus. Bimolecular fluorescence complementation assays showed that AtCyp19-3 interacts with CaM in vivo also, thus, validating the in vitro observations. However, the PPIase activity of the Arabidopsis cyclophilin was not affected by CaM. The implications of these findings are discussed in the context of Ca(2+) signaling and cyclophilin activity in Arabidopsis.
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