Evidence map›Paper›PMID 26316011›Full record

ArticleStem cell research & therapy2015

Stem cells isolated from human dental pulp and amniotic fluid improve skeletal muscle histopathology in mdx/SCID mice.

Alessandra Pisciotta, Massimo Riccio, Gianluca Carnevale, Aiping Lu, Sara De Biasi, Lara Gibellini, Giovanni B La Sala, Giacomo Bruzzesi, Adriano Ferrari, Johnny Huard and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.

0numbers the graph read from it
0cells of the map it votes in
42citing papers in PubMed
3.1field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

42 citing papers in PubMed, 76 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 2 countries.

Alessandra PisciottaDepartment of Surgical, Medical, Dental and Morphological Sciences with interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, via del Pozzo 71, 41124, Modena, Italy. alessandra.pisciotta@unimore.it.
Massimo RiccioDepartment of Surgical, Medical, Dental and Morphological Sciences with interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, via del Pozzo 71, 41124, Modena, Italy. massimo.riccio@unimore.it.
Gianluca CarnevaleDepartment of Surgical, Medical, Dental and Morphological Sciences with interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, via del Pozzo 71, 41124, Modena, Italy. gianluca.carnevale@unimore.it.
Aiping LuStem Cell Research Center, Department of Orthopaedic Surgery, University of Pittsburgh, 450 Technology Drive, Bridgeside Point II, Suite 206, 15219, Pittsburgh, PA, USA. ail3@pitt.edu.
Sara De BiasiDepartment of Surgical, Medical, Dental and Morphological Sciences with interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, via del Pozzo 71, 41124, Modena, Italy. sara.debiasi@unimore.it.
Lara GibelliniDepartment of Surgical, Medical, Dental and Morphological Sciences with interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, via del Pozzo 71, 41124, Modena, Italy. lara.gibellini@unimore.it.
Giovanni B La SalaDepartment of Obstetrics and Gynecology, Arcispedale Santa Maria Nuova, viale Risorgimento 80, 42123, Reggio Emilia, Italy. lasala.giovanni@asmn.re.it.
Giacomo BruzzesiOro-Maxillo-Facial Department, AUSL Baggiovara, via Giardini 1355, 41126, Modena, Baggiovara, Italy. gbruzzesi@libero.it.
Adriano FerrariDepartment of Biomedical, Metabolic and Neuroscience, University of Modena and Reggio Emilia, Children Rehabilitation Special Unit, IRCCS Arcispedale Santa Maria Nuova, viale Risorgimento 80, 42123, Reggio Emilia, Italy. adriano.ferrari@unimore.it.
Johnny HuardStem Cell Research Center, Department of Orthopaedic Surgery, University of Pittsburgh, 450 Technology Drive, Bridgeside Point II, Suite 206, 15219, Pittsburgh, PA, USA. jhuard@pitt.edu.
Anto De PolDepartment of Surgical, Medical, Dental and Morphological Sciences with interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, via del Pozzo 71, 41124, Modena, Italy. anto.depol@unimore.it.
University of Modena and Reggio Emilia · ITPoint Park University · USNational Operating Committee on Standards for Athletic Equipment · USOspedale Santa Maria · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionDuchenne muscular dystrophy (DMD), caused by a lack of the functional structural protein dystrophin, leads to severe muscle degeneration where the patients are typically wheelchair-bound and die in their mid-twenties from cardiac or respiratory failure or both. The aim of this study was to investigate the potential of human dental pulp stem cells (hDPSCs) and human amniotic fluid stem cells (hAFSCs) to differentiate toward a skeletal myogenic lineage using several different protocols in order to determine the optimal conditions for achieving myogenic commitment and to subsequently evaluate their contribution in the improvement of the pathological features associated with dystrophic skeletal muscle when intramuscularly injected into mdx/SCID mice, an immune-compromised animal model of DMD.

methodsHuman DPSCs and AFSCs were differentiated toward myogenic lineage in vitro through the direct co-culture with a myogenic cell line (C2C12 cells) and through a preliminary demethylation treatment with 5-Aza-2'-deoxycytidine (5-Aza), respectively. The commitment and differentiation of both hDPSCs and hAFSCs were evaluated by immunofluorescence and Western blot analysis. Subsequently, hDPSCs and hAFSCs, preliminarily demethylated and pre-differentiated toward a myogenic lineage for 2 weeks, were injected into the dystrophic gastrocnemius muscles of mdx/SCID mice. After 1, 2, and 4 weeks, the gastrocnemius muscles were taken for immunofluorescence and histological analyses.

resultsBoth populations of cells engrafted within the host muscle of mdx/SCID mice and through a paracrine effect promoted angiogenesis and reduced fibrosis, which eventually led to an improvement of the histopathology of the dystrophic muscle.

conclusionThis study shows that hAFSCs and hDPSCs represent potential sources of stem cells for translational strategies to improve the histopathology and potentially alleviate the muscle weakness in patients with DMD.

Indexed as

Mesenchymal Stem Cell TransplantationMuscle DevelopmentAmniotic FluidAnimalsCell LineDental PulpHumansMesenchymal Stem CellsMiceMice, Inbred mdxMice, SCIDMuscle, SkeletalMuscular Dystrophy, DuchenneRegeneration

Identifiers

PMID26316011
PMCPMC4552417
OpenAlexW1913981912

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.