Evidence map›Paper›PMID 26315820›Full record

ArticleProtoplasma2016

Advances in Boerhaavia diffusa hairy root technology: a valuable pursuit for identifying strain sensitivity and up-scaling factors to refine metabolite yield and bioactivity potentials.

Ruby Gupta, Pallavi Pandey, Sailendra Singh, Dhananjay Kumar Singh, Archana Saxena, Suaib Luqman, Dnyaneshwar U Bawankule, Suchitra Banerjee

Abstract read
PubMed Publisher
In one paragraph

Article in Protoplasma, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.6field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 24 citations in OpenAlex.

  1. Article
  2. Review
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  4. Review
  5. Influence of different3 Biotech · 2018
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Ruby GuptaPlant Biotechnology Department, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Lucknow, 226015, Uttar Pradesh, India.
Pallavi PandeyPlant Biotechnology Department, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Lucknow, 226015, Uttar Pradesh, India.
Sailendra SinghPlant Biotechnology Department, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Lucknow, 226015, Uttar Pradesh, India.
Dhananjay Kumar SinghMolecular Bioprospection Division, Central Institute of Medicinal and Aromatic Plants (CSIR- CIMAP), Lucknow, 226015, Uttar Pradesh, India.
Archana SaxenaMolecular Bioprospection Division, Central Institute of Medicinal and Aromatic Plants (CSIR- CIMAP), Lucknow, 226015, Uttar Pradesh, India.
Suaib LuqmanMolecular Bioprospection Division, Central Institute of Medicinal and Aromatic Plants (CSIR- CIMAP), Lucknow, 226015, Uttar Pradesh, India.
Dnyaneshwar U BawankuleMolecular Bioprospection Division, Central Institute of Medicinal and Aromatic Plants (CSIR- CIMAP), Lucknow, 226015, Uttar Pradesh, India.
Suchitra BanerjeePlant Biotechnology Department, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Lucknow, 226015, Uttar Pradesh, India. suchitrabanerjee07@yahoo.com.
Central Institute of Medicinal and Aromatic Plants · INAcademy of Scientific and Innovative Research · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The present study reports the Agrobacterium rhizogenes-mediated hairy root induction in of an ethno-medicinally significant herb-Boerhaavia diffusa L., for elucidating the underlying competence regarding its biosynthetic (i.e. boeravinone B and eupalitin) and bioactivity (antibacterial, antioxidant and anti-inflammatory) potentials. Host plant-specific receptiveness towards A. rhizogenes strains and disparity in compatibility threshold of leaf and nodal explants were evident. Only leaf explants responded, attaining hairy root induction with the ATCC 15834 followed by A4 and SA79 strains in reducing order of transformation efficiency. The growth behaviours differed amongst independent rhizoclones, and two clones of A4 (RBH) and ATCC 15834 (RBT8) origin demonstrated higher growth potentials. Polymerase chain reaction amplification of rol genes confirmed their transformed nature. Optimization of the appropriate solvent and reverse phase high-performance liquid chromatography parameters relating to the targeted metabolite production in the selected RBH and RBT8 clones revealed higher accumulation of eupalitin with the RBH clone having the best result of 1.44 times greater yield over the control root. Compared to the selected rhizoclones, the control roots however showed higher boeravinone B content. Devising a modified "stirred-tank" reactor through equipping with marine impellers and ring spargers facilitated high-density RBH root biomass yield with 6.1-fold and 1.15-fold yield increment of the boeravinone B and eupalitin respectively compared to shake-flask cultures. Considering the control roots, the RBH clone revealed analogous antioxidant/antibacterial activities with improved anti-inflammatory potential. The hairy root mediated higher production of boeravinone B and eupalitin could be achieved for the first time in bioreactor.

Indexed as

AgrobacteriumAnimalsAnti-Bacterial AgentsAntioxidantsBioreactorsCells, CulturedEscherichia coliFlavonoidsHumansLipopolysaccharidesMacrophagesMicrobial Sensitivity TestsNyctaginaceaePlant ExtractsPlant LeavesPlant ProteinsAnti-Bacterial AgentsAntioxidantsboeravinone BFlavonoidsLipopolysaccharidesPlant ExtractsPlant ProteinsA. rhizogenes strainsBioactivitiesBioreactorBoerhaavia diffusaHairy rootsMetabolites

Identifiers

PMID26315820
OpenAlexW1907861700

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.