ArticlePloS one2015
Genome-Wide Identification and Validation of Reference Genes in Infected Tomato Leaves for Quantitative RT-PCR Analyses.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 50 citations in OpenAlex.
- Genome-Wide Analysis of theInternational journal of molecular sciences · 2026Article
- Optimizing reference gene selection for accurate gene expression analysis in plants.Plant signaling & behavior · 2025Review
- Transcriptome-Based Identification of Reference Genes for Expression Analysis in Cassava UnderPlants (Basel, Switzerland) · 2025Article
- Key regulatory genes in sugar beet's defense against curly top virus identified by network analysis and qRT-PCR.Biochemistry and biophysics reports · 2025Article
- Chloroplast Vesiculation and InducedPlant direct · 2025Article
- A stable combination of non-stable genes outperforms standard reference genes for RT-qPCR data normalization.Scientific reports · 2024Article
- Genetic Analysis of the Peach SnRK1β3 Subunit and Its Function in Transgenic Tomato Plants.Genes · 2024Article
- Editing of SlWRKY29 by CRISPR-activation promotes somatic embryogenesis in Solanum lycopersicum cv. Micro-Tom.PloS one · 2024Article
- Global expression patterns ofFrontiers in plant science · 2023Article
- Not Only Systemin: Prosystemin Harbors Other Active Regions Able to Protect Tomato Plants.Frontiers in plant science · 2022Article
- Selection and Evaluation of Candidate Reference Genes for Quantitative Real-Time PCR in Aboveground Tissues and Drought Conditions inFrontiers in genetics · 2022Article
- Genome-wide screening of novel RT-qPCR reference genes for study of GLRaV-3 infection in wine grapes and refinement of an RNA isolation protocol for grape berries.Plant methods · 2021Article
- Stability analysis of reference genes for RT-qPCR assays involving compatible and incompatible Ralstonia solanacearum-tomato 'Hawaii 7996' interactions.Scientific reports · 2021Article
- Validation of Reference Genes for Quantitative Real-Time PCR Normalization inFrontiers in genetics · 2021Article
- Humic Acid Enhances the Growth of Tomato Promoted by Endophytic Bacterial Strains Through the Activation of Hormone-, Growth-, and Transcription-Related Processes.Frontiers in plant science · 2020Article
- Tomato Plants Treated with Systemin Peptide Show Enhanced Levels of Direct and Indirect Defense Associated with Increased Expression of Defense-Related Genes.Plants (Basel, Switzerland) · 2019Article
- Transcriptome-based identification and validation of reference genes for plant-bacteria interaction studies using Nicotiana benthamiana.Scientific reports · 2019Article
- Transcriptome and Metabolome Reprogramming in Tomato Plants byFrontiers in physiology · 2019Article
- Evaluation of internal control genes for quantitative realtime PCR analyses for studying fruit development of dwarf tomato cultivar 'Micro-Tom'.Plant biotechnology (Tokyo, Japan) · 2018Article
- The pattern of 1-aminocyclopropane-1-carboxylate oxidase induction in the tomato leaf petiole abscission zone is independent of expression of the ribonuclease-LX-encoding LeLX gene.Plant biology (Stuttgart, Germany) · 2018Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Gram-negative bacterium Xanthomonas campestris pv. vesicatoria (Xcv) causes bacterial spot disease of pepper and tomato by direct translocation of type III effector proteins into the plant cell cytosol. Once in the plant cell the effectors interfere with host cell processes and manipulate the plant transcriptome. Quantitative RT-PCR (qRT-PCR) is usually the method of choice to analyze transcriptional changes of selected plant genes. Reliable results depend, however, on measuring stably expressed reference genes that serve as internal normalization controls. We identified the most stably expressed tomato genes based on microarray analyses of Xcv-infected tomato leaves and evaluated the reliability of 11 genes for qRT-PCR studies in comparison to four traditionally employed reference genes. Three different statistical algorithms, geNorm, NormFinder and BestKeeper, concordantly determined the superiority of the newly identified reference genes. The most suitable reference genes encode proteins with homology to PHD finger family proteins and the U6 snRNA-associated protein LSm7. In addition, we identified pepper orthologs and validated several genes as reliable normalization controls for qRT-PCR analysis of Xcv-infected pepper plants. The newly identified reference genes will be beneficial for future qRT-PCR studies of the Xcv-tomato and Xcv-pepper pathosystems, as well as for the identification of suitable normalization controls for qRT-PCR studies of other plant-pathogen interactions, especially, if related plant species are used in combination with bacterial pathogens.
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