ArticleNature protocols2015
Direct metabolomics for plant cells by live single-cell mass spectrometry.
Article in Nature protocols, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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Who cites it
82 citing papers in PubMed.
- Single-Cell and Spatial Omics Technologies in Rice Abiotic Stress Biology: A Methodological Review.International journal of molecular sciences · 2026Review
- Imaging-Guided Live Single-Cell Lipid Profiling of Leader and Follower Cells During Collective Migration of Triple-Negative Breast Cancer Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Living single-cell metabolomicsChemical science · 2026Review
- Advanced technologies of single-cell metabolomics unveiling cellular metabolic heterogeneity for biological and biomedical research.Journal of food and drug analysis · 2026Review
- Single-Cell and Spatial Omics: Methods and Applications.MedComm · 2026Review
- Comparison of Liquid Chromatography- and Nano-Electrospray Ionization-Mass Spectrometry Approaches for Single-Cell Metabolomics.Analytical chemistry · 2026Article
- Metabolomics using anion-exchange chromatography mass spectrometry for the analysis of cells, tissues and biofluids.Nature protocols · 2026Review
- Evolution of petal patterning: blooming floral diversity at the microscale.The New phytologist · 2025Review
- Evaluating inkjet dispenser/liquid vortex capture-mass spectrometry for single-cell metabolomics in Hep G2 steatosis caused by tamoxifen.Analytical and bioanalytical chemistry · 2025Article
- Delineation of Subcellular Molecular Heterogeneity in Single Cells via Ultralow-Flow-Rate Desorption Electrospray Ionization Mass Spectrometry Imaging.Analytical chemistry · 2025Article
- Phenotype microarray-based assessment of metabolic variability in plant protoplasts.Plant methods · 2025Article
- Exploring Single-Probe Single-Cell Mass Spectrometry: Current Trends and Future Directions.Analytical chemistry · 2025Review
- Unravelling lipid heterogeneity: Advances in single-cell lipidomics in cellular metabolism and disease.BBA advances · 2025Review
- Single-cell omics: experimental workflow, data analyses and applications.Science China. Life sciences · 2025Review
- SCMeTA: a pipeline for single-cell metabolic analysis data processing.Bioinformatics (Oxford, England) · 2024Article
- Spatial single-cell isotope tracing reveals heterogeneity of de novo fatty acid synthesis in cancer.Nature metabolism · 2024Article
- Quantitative Single-Cell Mass Spectrometry Provides a Highly Resolved Analysis of Natural Product Biosynthesis Partitioning in Plants.Journal of the American Chemical Society · 2024Article
- Development of a highly efficient solubilization method for mass spectrometric analysis of phospholipids in living single cells.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2024Article
- Article
- Plant biotechnology research with single-cell transcriptome: recent advancements and prospects.Plant cell reports · 2024Review
22 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Live single-cell mass spectrometry (live MS) provides a mass spectrum that shows thousands of metabolite peaks from a single live plant cell within minutes. By using an optical microscope, a cell is chosen for analysis and a metal-coated nanospray microcapillary tip is used to remove the cell's contents. After adding a microliter of ionization solvent to the opposite end of the tip, the trapped contents are directly fed into the mass spectrometer by applying a high voltage between the tip and the inlet port of the spectrometer to induce nanospray ionization. Proteins are not detected because of insufficient sensitivity. Metabolite peaks are identified by exact mass or tandem mass spectrometry (MS/MS) analysis, and isomers can be separated by combining live MS with ion-mobility separation. By using this approach, spectra can be acquired in 10 min. In combination with metabolic maps and/or molecular databases, the data can be annotated into metabolic pathways; the data analysis takes 30 min to 4 h, depending on the MS/MS data availability from databases. This method enables the analysis of a number of metabolites from a single cell with rapid sampling at sub-attomolar-level sensitivity.
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Registered trials
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