ArticlePloS one2015
Multifactor Regulation of the MdtJI Polyamine Transporter in Shigella.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 26 citations in OpenAlex.
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- Still rocking in the structural era: A molecular overview of the small multidrug resistance (SMR) transporter family.The Journal of biological chemistry · 2022Review
- The VirF21:VirF30 protein ratio is affected by temperature and impacts Shigella flexneri host cell invasion.FEMS microbiology letters · 2022Article
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- Modulation of OMV Production by the Lysis Module of the DLP12 Defective Prophage ofMicroorganisms · 2021Article
- Host - Bacterial Pathogen Communication: The Wily Role of the Multidrug Efflux Pumps of the MFS Family.Frontiers in molecular biosciences · 2021Review
- The Great ESKAPE: Exploring the Crossroads of Bile and Antibiotic Resistance in Bacterial Pathogens.Infection and immunity · 2020Review
- Expression Profile of Multidrug Resistance Efflux Pumps During Intracellular Life of Adherent-InvasiveFrontiers in microbiology · 2020Article
- A Novel sRNA inFrontiers in cellular and infection microbiology · 2020Article
- The Varied Role of Efflux Pumps of the MFS Family in the Interplay of Bacteria with Animal and Plant Cells.Microorganisms · 2019Review
- The MFS efflux pump EmrKY contributes to the survival of Shigella within macrophages.Scientific reports · 2019Article
- Article
- Mechanisms of Bacterial Tolerance and Persistence in the Gastrointestinal and Respiratory Environments.Clinical microbiology reviews · 2018Review
- Pulmonary Surfactant Promotes Virulence Gene Expression and Biofilm Formation in Klebsiella pneumoniae.Infection and immunity · 2018Article
- Generation of a platform strain for ionic liquid tolerance using adaptive laboratory evolution.Microbial cell factories · 2017Article
- The Intriguing Evolutionary Journey of EnteroinvasiveFrontiers in microbiology · 2017Review
- A Novel Putrescine Exporter SapBCDF of Escherichia coli.The Journal of biological chemistry · 2016Article
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The polyamine profile of Shigella, the etiological agent of bacillary dysentery in humans, differs markedly from that of E. coli, its innocuous commensal ancestor. Pathoadaptive mutations such as the loss of cadaverine and the increase of spermidine favour the full expression of the virulent phenotype of Shigella. Spermidine levels affect the expression of the MdtJI complex, a recently identified efflux pump belonging to the small multi-drug resistance family of transporters. In the present study, we have addressed the regulation of the mdtJI operon in Shigella by asking which factors influence its expression as compared to E. coli. In particular, after identifying the mdtJI promoter by primer extension analysis, in vivo transcription assays and gel-retardation experiments were carried out to get insight on the silencing of mdtJI in E. coli. The results indicate that H-NS, a major nucleoid protein, plays a key role in repressing the mdtJI operon by direct binding to the regulatory region. In the Shigella background mdtJI expression is increased by the high levels of spermidine typically found in this microorganism and by VirF, the plasmid-encoded regulator of the Shigella virulence regulatory cascade. We also show that the expression of mdtJI is stimulated by bile components. Functional analyses reveal that MdtJI is able to promote the excretion of putrescine, the spermidine precursor. This leads us to consider the MdtJI complex as a possible safety valve allowing Shigella to maintain spermidine to a level optimally suited to survival within infected macrophages and, at the same time, prevent toxicity due to spermidine over-accumulation.
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