ArticleThe open orthopaedics journal2015
In Vivo Bioluminescence Imaging - A Suitable Method to Track Mesenchymal Stromal Cells in a Skeletal Muscle Trauma.
Article in The open orthopaedics journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 16 citations in OpenAlex.
- Article
- Article
- Comparison of concentrated fresh mononuclear cells and cultured mesenchymal stem cells from bone marrow for bone regeneration.Stem cells translational medicine · 2021Article
- Stem cell homing, tracking and therapeutic efficiency evaluation for stroke treatment using nanoparticles: A systematic review.World journal of stem cells · 2020Article
- Fate of systemically and locally administered adipose-derived mesenchymal stromal cells and their effect on wound healing.Stem cells translational medicine · 2020Article
- (Epi)genetic Modifications in Myogenic Stem Cells: From Novel Insights to Therapeutic Perspectives.Cells · 2019Review
- The Complement System Is Essential for the Phagocytosis of Mesenchymal Stromal Cells by Monocytes.Frontiers in immunology · 2019Article
- Co-transplantation of mesenchymal stem cells improves spermatogonial stem cell transplantation efficiency in mice.Stem cell research & therapy · 2018Article
- Unraveling local tissue changes within severely injured skeletal muscles in response to MSC-based intervention using MALDI Imaging mass spectrometry.Scientific reports · 2018Article
- The influence of systemically or locally administered mesenchymal stem cells on tissue repair in a rat oral implantation model.International journal of implant dentistry · 2018Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelledCell-based therapies have emerged during the last decade in various clinical fields. Especially mesenchymal stromal cells (MSCs) have been used in pre-clinical and clinical applications in cardiovascular, neurodegenerative and musculoskeletal disorders. In order to validate survival and viability as well as possible engraftment of MSCs into the host tissue a live cell imaging technique is needed that allows non-invasive, temporal imaging of cellular kinetics as well as evaluation of cell viability after transplantation. In this study we used luciferase-based bioluminescence imaging (BLI) to investigate the survival of autologous MSCs transplanted into a severely crushed soleus muscle of the rats. Furthermore we compared local as well as intra-arterial (i.a.) administration of cells and analyzed if luciferase transduced MSCs depict the same characteristics in vitro as non-transduced MSCs. We could show that transduction of MSCs does not alter their in vitro characteristics, thus, transduced MSCs display the same differentiation, proliferation and migration capacity as non-transduced cells. Using BLI we could track MSCs transplanted into a crushed soleus muscle until day 7 irrespective of local or i.a. APPLICATION: Hence, our study proves that luciferase-based BLI is a suitable method for in vivo tracking of MSCs in skeletal muscle trauma in rats.
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