ArticleProceedings of the National Academy of Sciences of the United States of America2015
In situ structural analysis of Golgi intracisternal protein arrays.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
45 citing papers in PubMed, 114 citations in OpenAlex.
- Human Proteoglycan Linkage Region Glycosyltransferases are Dimeric and Show Unexpected Specificities.Angewandte Chemie (International ed. in English) · 2026Article
- Targeted imaging of specialized plant cell walls by improved cryo-CLEM and cryo-electron tomography.Nature communications · 2025Article
- Quantitative intra-Golgi transport and organization data suggest the stable compartment nature of the Golgi.eLife · 2025Article
- Structural glycobiology - from enzymes to organelles.Biochemical Society transactions · 2025Review
- A century journey of organelles research in the plant endomembrane system.The Plant cell · 2024Review
- The application and development of electron microscopy for three-dimensional reconstruction in life science: a review.Cell and tissue research · 2024Review
- Rendering protein structures inside cells at the atomic level with Unreal Engine.bioRxiv : the preprint server for biology · 2024Article
- Bringing Structure to Cell Biology with Cryo-Electron Tomography.Annual review of biophysics · 2023Review
- A non-canonical role of ATG8 in Golgi recovery from heat stress in plants.Nature plants · 2023Article
- PreparingFrontiers in plant science · 2023Article
- Contribution of the Golgi apparatus in morphogenesis of a virus-induced cytopathic vacuolar system.Life science alliance · 2022Article
- Electron microscopy for imaging organelles in plants and algae.Plant physiology · 2022Article
- A glossary of plant cell structures: Current insights and future questions.The Plant cell · 2022Review
- Imaging Cytoskeleton Components by Electron Microscopy.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Intracellular nanoscale architecture as a master regulator of calcium carbonate crystallization in marine microalgae.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Challenges and triumphs in cryo-electron tomography.iScience · 2021Review
- Membrane imaging in the plant endomembrane system.Plant physiology · 2021Article
- DNA origami signposts for identifying proteins on cell membranes by electron cryotomography.Cell · 2021Article
- An introduction to cryo-FIB-SEM cross-sectioning of frozen, hydrated Life Science samples.Journal of microscopy · 2021Article
- Visualizing insulin vesicle neighborhoods in β cells by cryo-electron tomography.Science advances · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
We acquired molecular-resolution structures of the Golgi within its native cellular environment. Vitreous Chlamydomonas cells were thinned by cryo-focused ion beam milling and then visualized by cryo-electron tomography. These tomograms revealed structures within the Golgi cisternae that have not been seen before. Narrow trans-Golgi lumina were spanned by asymmetric membrane-associated protein arrays that had ∼6-nm lateral periodicity. Subtomogram averaging showed that the arrays may determine the narrow central spacing of the trans-Golgi cisternae through zipper-like interactions, thereby forcing cargo to the trans-Golgi periphery. Additionally, we observed dense granular aggregates within cisternae and intracisternal filament bundles associated with trans-Golgi buds. These native in situ structures provide new molecular insights into Golgi architecture and function.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.