ArticleBMC genomics2015
Haemophilus influenzae: using comparative genomics to accurately identify a highly recombinogenic human pathogen.
Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- SputumInternational journal of chronic obstructive pulmonary disease · 2019Trial
- Patients with Chronic Obstructive Pulmonary Disease harbour a variation of Haemophilus species.Scientific reports · 2018Trial
- CNN4Essential: a convolutional neural network model for predicting bacterial gene essentiality based on multi-feature fusion.BMC genomics · 2026Article
- Mucosal immune cell priming by intranasally deliveredFrontiers in immunology · 2026Article
- Genetic population structure of Haemophilus influenzae at local and global scales.Nature microbiology · 2025Article
- Molecular insights into pangenome localization and constructs design for Hemophilus influenza vaccine.Scientific reports · 2025Article
- Optimizing recovery of Haemophilus influenzae from vaginal-rectal specimens and determining carriage rates in pregnant women.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024Article
- Rare serotype c Haemophilus influenzae invasive isolate: characterization of the first case in Portugal.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024Article
- Otitis media: recent advances in otitis media vaccine development and model systems.Frontiers in microbiology · 2024Review
- Genomic classification and antimicrobial resistance profiling of Streptococcus pneumoniae and Haemophilus influenza isolates associated with paediatric otitis media and upper respiratory infection.BMC infectious diseases · 2023Article
- Comparative Genomic Analysis Reveals Genetic Diversity and Pathogenic Potential of Haemophilus seminalis and Emended Description of Haemophilus seminalis.Microbiology spectrum · 2023Article
- Antigen-specific memory Th17 cells promote cross-protection against nontypeable Haemophilus influenzae after mild influenza A virus infection.Mucosal immunology · 2023Article
- Can non-typeablePediatric investigation · 2023Article
- Direct Real-Time PCR for the Detection and Serotyping of Haemophilus influenzae without DNA Extraction.Journal of clinical microbiology · 2022Article
- Whole genome sequencing-based classification of human-related Haemophilus species and detection of antimicrobial resistance genes.Genome medicine · 2022Article
- An invasive Haemophilus influenzae serotype b infection in an Anglo-Saxon plague victim.Genome biology · 2022Article
- It Takes Two to Tango: Combining Conventional Culture With Molecular Diagnostics Enhances Accuracy ofFrontiers in microbiology · 2022Article
- Predominant Bacterial and Viral Otopathogens Identified Within the Respiratory Tract and Middle Ear of Urban Australian Children Experiencing Otitis Media Are Diversely Distributed.Frontiers in cellular and infection microbiology · 2022Article
- Lipoprotein(a), an Opsonin, Enhances the Phagocytosis of NontypeableJournal of immunology research · 2021Article
- Learning from -omics strategies applied to uncoverComputational and structural biotechnology journal · 2021Review
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHaemophilus influenzae is an opportunistic bacterial pathogen that exclusively colonises humans and is associated with both acute and chronic disease. Despite its clinical significance, accurate identification of H. influenzae is a non-trivial endeavour. H. haemolyticus can be misidentified as H. influenzae from clinical specimens using selective culturing methods, reflecting both the shared environmental niche and phenotypic similarities of these species. On the molecular level, frequent genetic exchange amongst Haemophilus spp. has confounded accurate identification of H. influenzae, leading to both false-positive and false-negative results with existing speciation assays.
resultsWhole-genome single-nucleotide polymorphism data from 246 closely related global Haemophilus isolates, including 107 Australian isolate genomes generated in this study, were used to construct a whole-genome phylogeny. Based on this phylogeny, H. influenzae could be differentiated from closely related species. Next, a H. influenzae-specific locus, fucP, was identified, and a novel TaqMan real-time PCR assay targeting fucP was designed. PCR specificity screening across a panel of clinically relevant species, coupled with in silico analysis of all species within the order Pasteurellales, demonstrated that the fucP assay was 100 % specific for H. influenzae; all other examined species failed to amplify.
conclusionsThis study is the first of its kind to use large-scale comparative genomic analysis of Haemophilus spp. to accurately delineate H. influenzae and to identify a species-specific molecular signature for this species. The fucP assay outperforms existing H. influenzae targets, most of which were identified prior to the next-generation genomics era and thus lack validation across a large number of Haemophilus spp. We recommend use of the fucP assay in clinical and research laboratories for the most accurate detection and diagnosis of H. influenzae infection and colonisation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.