ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2016
Effective disposal of nitrogen waste in blood-fed Aedes aegypti mosquitoes requires alanine aminotransferase.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 20 citations in OpenAlex.
- Crosstalk in the cold: host-microbe interactions in insect diapause.Annals of the Entomological Society of America · 2026Review
- Regulatory mechanisms of nitrogen homeostasis in insect growth and development.Insect science · 2026Review
- Pyruvate kinase is post-translationally regulated by sirtuin 2 in Aedes aegypti mosquitoes.Insect biochemistry and molecular biology · 2023Article
- Ornithine decarboxylase deficiency critically impairs nitrogen metabolism and survival in Aedes aegypti mosquitoes.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- Untargeted metabolomics-based response analysis of temperature and insecticide exposure in Aedes aegypti.Scientific reports · 2022Article
- Unraveling mosquito metabolism with mass spectrometry-based metabolomics.Trends in parasitology · 2021Review
- The Multifaceted Roles of Proline in Cell Behavior.Frontiers in cell and developmental biology · 2021Review
- Mass spectrometry-based stable-isotope tracing uncovers metabolic alterations in pyruvate kinase-deficient Aedes aegypti mosquitoes.Insect biochemistry and molecular biology · 2020Article
- Article
- Development of Aedes aegypti (Diptera: Culicidae) mosquito larvae in high ammonia sewage in septic tanks causes alterations in ammonia excretion, ammonia transporter expression, and osmoregulation.Scientific reports · 2019Article
- Metabolic balancing by miR-276 shapes the mosquito reproductive cycle and Plasmodium falciparum development.Nature communications · 2019Article
- Identification and characterization of a mosquito-specific eggshell organizing factor in Aedes aegypti mosquitoes.PLoS biology · 2019Article
- Distinctive regulatory properties of pyruvate kinase 1 from Aedes aegypti mosquitoes.Insect biochemistry and molecular biology · 2019Article
- Positional stable isotope tracer analysis reveals carbon routes during ammonia metabolism ofFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- How micronutrients influence the physiology of mosquitoes.Current opinion in insect science · 2017Review
- Xanthine dehydrogenase-1 silencing inFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
To better understand the mechanisms responsible for the success of female mosquitoes in their disposal of excess nitrogen, we investigated the role of alanine aminotransferase (ALAT) in blood-fed Aedes aegypti. Transcript and protein levels from the 2 ALAT genes were analyzed in sucrose- and blood-fed A. aegypti tissues. ALAT1 and ALAT2 exhibit distinct expression patterns in tissues during the first gonotrophic cycle. Injection of female mosquitoes with either double-stranded RNA (dsRNA)-ALAT1 or dsRNA ALAT2 significantly decreased mRNA and protein levels of ALAT1 or ALAT2 in fat body, thorax, and Malpighian tubules compared with dsRNA firefly luciferase-injected control mosquitoes. The silencing of either A. aegypti ALAT1 or ALAT2 caused unexpected phenotypes such as a delay in blood digestion, a massive accumulation of uric acid in the midgut posterior region, and a significant decrease of nitrogen waste excretion during the first 48 h after blood feeding. Concurrently, the expression of genes encoding xanthine dehydrogenase and ammonia transporter (Rhesus 50 glycoprotein) were significantly increased in tissues of both ALAT1- and ALAT2-deficient females. Moreover, perturbation of ALAT1 and ALAT2 in the female mosquitoes delayed oviposition and reduced egg production. These novel findings underscore the efficient mechanisms that blood-fed mosquitoes use to avoid ammonia toxicity and free radical damage.-Mazzalupo, S., Isoe, J., Belloni, V., Scaraffia, P. Y. Effective disposal of nitrogen waste in blood-fed Aedes aegypti mosquitoes requires alanine aminotransferase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.