ArticleInternational journal of ophthalmology2015
Dectin-1 agonist curdlan modulates innate immunity to Aspergillus fumigatus in human corneal epithelial cells.
Article in International journal of ophthalmology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- Immunotherapy in Ocular Fungal Infections: Advances and Emerging Strategies.Current microbiology · 2026Review
- β-1,3-Glucan recognition byApplied and environmental microbiology · 2024Article
- Dectin-1 participates in the immune-inflammatory response to mouseFrontiers in immunology · 2024Article
- Dectin-1 Signaling Update: New Perspectives for Trained Immunity.Frontiers in immunology · 2022Review
- Epigenetic regulation of epithelial dectin-1 through an IL-33-STAT3 axis in allergic disease.Allergy · 2022Article
- HECT E3 Ubiquitin Ligase Nedd4 Is Required for Antifungal Innate Immunity.Journal of immunology (Baltimore, Md. : 1950) · 2021Article
- Coacervate Thermoresponsive Polysaccharide Nanoparticles as Delivery System for Piroxicam.International journal of molecular sciences · 2020Article
- Review
- Pattern recognition receptors in fungal immunity.Seminars in cell & developmental biology · 2019Review
- HIF1α-dependent glycolysis promotes macrophage functional activities in protecting against bacterial and fungal infection.Scientific reports · 2018Article
- Dectin-1-Mediated Pathway Contributes to Fusarium proliferatum-Induced CXCL-8 Release from Human Respiratory Epithelial Cells.International journal of molecular sciences · 2017Article
- E-cadherin mediates adhesion of Aspergillus fumigatus to non-small cell lung cancer cells.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- Review of clinical and basic approaches of fungal keratitis.International journal of ophthalmology · 2016Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo explore the immunomodulatory effects of curdlan on innate immune responses against Aspergillus fumigatus (A. fumigatus) in cultured human corneal epithelial cells (HCECs), and whether C-type lectin receptor Dectin-1 mediates the immunomodulatory effects of curdlan.
methodsThe HCECs were stimulated by curdlan in different concentrations (50, 100, 200, 400 µg/mL) for various time. Then HCECs pretreated with or without laminarin (Dectin-1 blocker, 0.3 mg/mL) and curdlan were stimulated by A. fumigatus hyphae. The mRNA and protein production of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were determined by real-time quantitative polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. The protein level of Dectin-1 was measured by Western blot.
resultsCurdlan stimulated mRNA expression of TNF-α and IL-6 in a dose and time dependent manner in HCECs. Curdlan pretreatment before A. fumigatus hyphae stimulation significantly enhanced the expression of TNF-α and IL-6 at mRNA and protein levels compared with A. fumigatus hyphae stimulation group (P<0.05). Both curdlan and A. fumigatus hyphae up-regulated Dectin-1 protein expression in HCECs, and Dectin-1 expression was elevated to 1.5- to 2-fold by curdlan pretreatment followed hyphae stimulation. The Dectin-1 blocker laminarin suppressed the mRNA expression and protein production of TNF-α and IL-6 induced by curdlan and hyphae (P<0.05).
conclusionThese findings demonstrated that curdlan pretreatment enhanced the inflammatory response induced by A. fumigatus hyphae in HCECs. Dectin-1 is essential for the immunomodulatory effects of curdlan. Curdlan may have high clinical application values in fungal keratitis treatment.
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