ArticleBioResearch open access2015
A Scalable Perfusion Culture System with Miniature Peristaltic Pumps for Live-Cell Imaging Assays with Provision for Microfabricated Scaffolds.
Article in BioResearch open access, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Incubator-Free Organoid Culture in a Sealed Recirculatory System.bioRxiv : the preprint server for biology · 2025Article
- Fast formation and maturation enhancement of human liver organoids using a liver-organoid-on-a-chip.Frontiers in cell and developmental biology · 2024Article
- Peptide-Functionalized Electrospun Meshes for the Physiological Cultivation of Pulmonary Alveolar Capillary Barrier Models in a 3D-Printed Micro-Bioreactor.ACS biomaterials science & engineering · 2023Article
- An automated modular open-technology device to measure and adjust concentration of aquatic sperm samples for cryopreservation.SLAS technology · 2023Article
- Utility of low-cost, miniaturized peristaltic and Venturi pumps in droplet microfluidics.Analytica chimica acta · 2021Article
- Survival Rate of Cells Sent by a Low Mechanical Load Tube Pump: The "Ring Pump".Micromachines · 2020Article
- Large scale and integrated platform for digital mass culture of anchorage dependent cells.Nature communications · 2019Article
- 3D printing in cell culture systems and medical applications.Applied physics reviews · 2018Review
- High density Huh7.5 cell hollow fiber bioreactor culture for high-yield production of hepatitis C virus and studies of antivirals.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present a perfusion culture system with miniature bioreactors and peristaltic pumps. The bioreactors are designed for perfusion, live-cell imaging studies, easy incorporation of microfabricated scaffolds, and convenience of operation in standard cell culture techniques. By combining with miniature peristaltic pumps-one for each bioreactor to avoid cross-contamination and to maintain desired flow rate in each-we have made a culture system that facilitates perfusion culture inside standard incubators. This scalable system can support multiple parallel perfusion experiments. The major components are fabricated by three-dimensional printing using VeroWhite, which we show to be amenable to ex vivo cell culture. Furthermore, the components of the system can be reused, thus making it economical. We validate the system and illustrate its versatility by culturing primary rat hepatocytes, live imaging the growth of mouse fibroblasts (NIH 3T3) on microfabricated ring-scaffolds inserted into the bioreactor, performing perfusion culture of breast cancer cells (MCF7), and high-magnification imaging of hepatocarcinoma cells (HuH7).
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Registered trials
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