Evidence map›Paper›PMID 26309790›Full record

ArticleBioResearch open access2015

Subcutaneous Adipose Tissue-Derived Stem Cell Utility Is Independent of Anatomical Harvest Site.

Mahmood S Choudhery, Michael Badowski, Angela Muise, John Pierce, David T Harris

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BioResearch open access, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
2.8field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 39 citations in OpenAlex.

  1. Article
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  10. [Clinical research progress of mesenchymal stem cells in treatment of chronic wounds].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2021
    Article
  11. Adipose Stem Cells in Regenerative Medicine: Looking Forward.Frontiers in bioengineering and biotechnology · 2021
    Review
  12. Review
  13. Article
  14. Article
  15. Article
  16. [Research progress of the donor factors and experimental factors affecting adipogenic differentiation of adipose derived stem cells].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2017
    Article
  17. Biological Therapies in Regenerative Sports Medicine.Sports medicine (Auckland, N.Z.) · 2017
    Review
  18. Article
  19. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Mahmood S ChoudheryTissue Engineering and Regenerative Medicine Laboratory, Advance Research Center of Biomedical Sciences, King Edward Medical University , Lahore, Pakistan . ; Department of Immunobiology, College of Medicine, The University of Arizona , Tucson, Arizona.
Michael BadowskiDepartment of Immunobiology, College of Medicine, The University of Arizona , Tucson, Arizona.
Angela MuiseDepartment of Immunobiology, College of Medicine, The University of Arizona , Tucson, Arizona.
John PierceAesthetic Surgery of Tucson , Tucson, Arizona.
David T HarrisDepartment of Immunobiology, College of Medicine, The University of Arizona , Tucson, Arizona.
University of Arizona · USTucson Medical Center · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

One of the challenges for tissue engineering and regenerative medicine is to obtain suitably large cell numbers for therapy. Mesenchymal stem cells (MSCs) can easily be expanded in vitro to obtain large numbers of cells, but this approach may induce cellular senescence. The characteristics of cells are dependent on variables like age, body mass index (BMI), and disease conditions, however, and in the case of adipose tissue-derived stem cells (ASCs), anatomical harvest site is also an important variable that can affect the regenerative potential of isolated cells. We therefore had kept the parameters (age, BMI, disease conditions) constant in this study to specifically assess influence of anatomical sites of individual donors on utility of ASCs. Adipose tissue was obtained from multiple anatomical sites in individual donors, and viability and nucleated cell yield were determined. MSC frequency was enumerated using colony forming unit assay and cells were characterized by flow cytometry. Growth characteristics were determined by long-term population doubling analysis of each sample. Finally, MSCs were induced to undergo adipogenic, osteogenic, and chondrogenic differentiation. To validate the findings, these results were compared with similar single harvest sites from multiple individual patients. The results of the current study indicated that MSCs obtained from multiple harvest sites in a single donor have similar morphology and phenotype. All adipose depots in a single donor exhibited similar MSC yield, viability, frequency, and growth characteristics. Equivalent differentiation capacity into osteocytes, adipocytes, and chondrocytes was also observed. On the basis of results, we conclude that it is acceptable to combine MSCs obtained from various anatomical locations in a single donor to obtain suitably large cell numbers required for therapy, avoiding in vitro senescence and lengthy and expensive in vitro culturing and expansion steps.

Indexed as

mesenchymal stem cellsmultiple harvest sitesregenerative potential

Identifiers

PMID26309790
PMCPMC4497709
OpenAlexW2316890337

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.