ArticleBioResearch open access2015
Induction of Osteogenic Differentiation in Human Mesenchymal Stem Cells by Crosstalk with Osteoblasts.
Article in BioResearch open access, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- A Scaffold-Based 3D Culture Model Including Selected Osteoprogenitors for Bone Regeneration With Controlled Morphology.Stem cells international · 2026Article
- Impact of different capping materials extracts on proliferation and osteogenic differentiation of cultured human dental pulp stem cells.Scientific reports · 2025Article
- Resolvin D1 improves allograft osteointegration and directly enhances osteoblasts differentiation.Frontiers in immunology · 2023Article
- The Combination of Platelet Rich Plasma Gel, Human Umbilical Mesenchymal Stem Cells and Nanohydroxyapatite/polyamide 66 Promotes Angiogenesis and Bone Regeneration in Large Bone Defect.Tissue engineering and regenerative medicine · 2022Article
- miRNA induced 3D bioprinted-heterotypic osteochondral interface.Biofabrication · 2022Article
- Article
- Chondrogenic differentiation of human bone marrow MSCs in osteochondral implants under kinematic mechanical load is dependent on the underlying osteo component.Frontiers in bioengineering and biotechnology · 2022Article
- Apheresis Platelet Rich-Plasma for Regenerative Medicine: An In Vitro Study on Osteogenic Potential.International journal of molecular sciences · 2021Article
- Ex Vivo Systems to Study Chondrogenic Differentiation and Cartilage Integration.Journal of functional morphology and kinesiology · 2021Review
- Cellular Fragments as Biomaterial for Rapid In Vitro Bone-Like Tissue Synthesis.International journal of molecular sciences · 2020Article
- Article
- Bone marrow mesenchymal stem cells: Aging and tissue engineering applications to enhance bone healing.Biomaterials · 2019Review
- Co-Culture with Human Osteoblasts and Exposure to Extremely Low Frequency Pulsed Electromagnetic Fields Improve Osteogenic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells.International journal of molecular sciences · 2018Article
- Biphasic Scaffolds from Marine Collagens for Regeneration of Osteochondral Defects.Marine drugs · 2018Article
- Translation of remote control regenerative technologies for bone repair.NPJ Regenerative medicine · 2018Article
- Osteogenic Programming of Human Mesenchymal Stem Cells with Highly Efficient Intracellular Delivery of RUNX2.Stem cells translational medicine · 2017Article
- Evaluation of the shape, viability, stemness and osteogenic differentiation of cell spheroids formed from human gingiva-derived stem cells and osteoprecursor cells.Experimental and therapeutic medicine · 2017Article
- The trans-well coculture of human synovial mesenchymal stem cells with chondrocytes leads to self-organization, chondrogenic differentiation, and secretion of TGFβ.Stem cell research & therapy · 2016Article
- Non-unions treated with bone morphogenic protein 7: introducing the quantitative measurement of human serum cytokine levels as promising tool in evaluation of adjunct non-union therapy.Journal of inflammation (London, England) · 2016Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Natural bone healing following fractures is initiated by osteoblasts (OBs) and mesenchymal stem cells (MSCs), a cell combination with possible potential in tissue engineering techniques for bony defects. The aim of the study was to investigate MSC/OB-crosstalk, in order to determine optimal cell culture conditions for osteogenic differentiation. Human OBs and MSCs interactions were investigated in an in vitro trans-well co-culture study over a time period of 28 days. Calcification was determined by optical density (OD) at 450 nm and Alizarin red staining. Messenger RNA expression was assessed by quantitative PCR. Osteogenic medium containing 1% fetal bovine serum resulted in superior levels of calcification in MSCs in co-culture with OBs compared to 2% or 5% fetal bovine serum (p<0.05). Comparing MSCs and OBs alone with the MSC/OB co-culture, calcification, as measured by OD 450 nm, increased over time in all groups. The highest values were recorded in the co-culture (p<0.05). Osteogenic differentiation potential showed significant interindividual differences. In order to predict differentiation potential, OD 450 nm measurements and mRNA expression of alkaline phosphatase were correlated with the population doubling rate during the expansion period. For OBs and MSCs, statistically significant associations of proliferation and differentiation potential were found (p<0.001). The addition of transforming growth factor beta resulted in up-regulation of collagen type I and Sp7 mRNA, and down-regulation of alkaline phosphatase mRNA. The results suggest the idea of soluble paracrine factors being secreted by OBs to induce osteogenic differentiation of MSCs.
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