Evidence map›Paper›PMID 26309572›Full record

ArticleInternational journal of clinical and experimental medicine2015

Construction and identification of Complex DNA vaccine of hepatitis B and Toxoplasma gondii.

Qing-Kuan Wei, Ting Xiao, Jin Li, Kun Yin, Feng-Ju Jia, Chao Xu, Gui-Hua Zhao, Yong Cui, Gong-Zhen Liu, Hui Sun and 3 more

Abstract read
PubMed Full text at PMC
In one paragraph

Article in International journal of clinical and experimental medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 99% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 1 institution in 1 country.

Qing-Kuan WeiShandong Academy of Medical Sciences, Shandong Institute of Parasitic Disease Jining 272033, China.
Ting XiaoShandong Academy of Medical Sciences, Shandong Institute of Parasitic Disease Jining 272033, China.
Jin LiShandong Academy of Medical Sciences, Shandong Institute of Parasitic Disease Jining 272033, China.
Kun YinShandong Academy of Medical Sciences, Shandong Institute of Parasitic Disease Jining 272033, China.
Feng-Ju JiaShandong Academy of Medical Sciences, Shandong Institute of Parasitic Disease Jining 272033, China.
Chao XuShandong Academy of Medical Sciences, Shandong Institute of Parasitic Disease Jining 272033, China.
Gui-Hua ZhaoShandong Academy of Medical Sciences, Shandong Institute of Parasitic Disease Jining 272033, China.
Yong CuiShandong Academy of Medical Sciences, Shandong Institute of Parasitic Disease Jining 272033, China.
Gong-Zhen LiuShandong Academy of Medical Sciences, Shandong Institute of Parasitic Disease Jining 272033, China.
Hui SunShandong Academy of Medical Sciences, Shandong Institute of Parasitic Disease Jining 272033, China.
Hong-Tao JiangShandong Academy of Medical Sciences, Shandong Institute of Parasitic Disease Jining 272033, China.
Ge YanShandong Academy of Medical Sciences, Shandong Institute of Parasitic Disease Jining 272033, China.
Bing-Cheng HuangShandong Academy of Medical Sciences, Shandong Institute of Parasitic Disease Jining 272033, China.
Jiangsu Institute of Parasitic Diseases · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo construct and identify multi-gene recombinant expression vector pcDNA3-HBsAg-p30-ROP2.

methodPrimers were designed according to the gene sequences of restriction enzyme cutting site of recombinant pcDNA3-p30-ROP2 and hepatitis B surface antigen (HBsAg). The target fragment of HBsAg was amplified and cloned to expression vector pcDNA3-p30-ROP2 by restriction enzyme digestion and ligation. The recombinant expression vector pcDNA3-HBsAg-p30-ROP2 was identified by PCR detection, followed by enzyme restriction and sequencing.

resultsThe target fragment of HBsAg was successfully amplified, and the multi-gene eukaryotic expression vector pcDNA3-HBsAg-p30-ROP2 was established. PCR detection and restriction enzyme digestion showed that the length of the target fragment was consistent with the theoretical value. The recombinant expression vector contained the complete sequences of p30-ROP2 compound gene and HBsAg.

conclusionMulti-gene recombinant expression vector pcDNA3-HBsAg-p30-ROP2 was successfully established. The constructed expression vector could be used to develop multi-gene nucleic acid vaccines.

Indexed as

genetic recombinationHBsAgRhoptry protein 2 (ROP2)surface antigen 1 (p30)Toxoplasma gondii

Identifiers

PMID26309572
PMCPMC4538168
OpenAlexW1180509323

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.