ArticleInternational journal of clinical and experimental medicine2015
Pre-B-cell colony enhancing factor (PBEF) increases endothelial permeability in hypoxia/re-oxygenation model.
Article in International journal of clinical and experimental medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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Who cites it
4 citing papers in PubMed, 6 citations in OpenAlex.
- Inhibition of Pre-B Cell Colony Enhancing Factor Reduces Lung Injury in Rats Receiving Cardiopulmonary Bypass.Drug design, development and therapy · 2021Article
- Pre-B-cell colony enhancing factor regulates the alveolar epithelial sodium-water transport system through the ERK and AKT pathways.American journal of translational research · 2019Article
- Promotion of anoxia-reoxygenation-induced inflammation and permeability enhancement by nicotinamide phosphoribosyltransferase-activated MAPK signaling in human umbilical vein endothelial cells.Experimental and therapeutic medicine · 2017Article
- Emodin alleviates severe acute pancreatitis-associated acute lung injury by decreasing pre-B-cell colony-enhancing factor expression and promoting polymorphonuclear neutrophil apoptosis.Molecular medicine reports · 2017Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThis study aims to explore the relationship between PBEF and VEGF and p-MLC and the mechanism of PBEF increasing permeability of endothelial cells in hypoxia/re-oxygenation.
methodsHypoxia/re-oxygenation model was established and PBEF siRNA was synthesized. According to the different HUVEC treatment, it can be divided into normal control group, PBEF siRNA group; hypoxia (20 hours) and re-oxygenation (3 h) group, hypoxia (20 h) and re-oxygenation (6 h) group, hypoxia (20 h) and re-oxygenation (9 hours) group, hypoxia (20 h)/re-oxygenation (12 h). The expressions of PBEF, VEGF and p-MLC were tested by RT-PCR and Western blot.
resultsThe mRNA and protein expression of PBEF in PBEF siRNA group were significantly lower compared to liposome group and the negative controls (P < 0.05). The expression of PBEF protein in hypoxia/re-oxygenation group was significantly higher than the normal control group. It increased in the 3 h of re-oxygenation group, peaked at 9 h, until 12 h started to decline (P < 0.05). When the PBEF gene was knockdown, the expression of VEGF and p-MLC in hypoxia and re-oxygenation are significantly lower.
conclusionsPBEF siRNA can effectively inhibit the expression of PBEF in endothelial cells. The expression of PBEF, VEGF and p-MLC were significantly higher in endothelial cell after Hypoxia/re-oxygenation. PBEF may change the permeability of endothelial cells by regulating the expression of VEGF and the phosphorylation of MLC.
Indexed as
Identifiers
26309537PMC4537969W1148370589What OpenQuestion holds
Registered trials
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