ArticlePloS one2015
Chemical Composition and In Vitro Cytotoxicity of Essential Oils from Leaves and Flowers of Callistemon citrinus from Western Himalayas.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 61 citations in OpenAlex.
- Antinociceptive Effects of Free and β-Cyclodextrin-Associated α-Phellandrene in CFA-Induced Inflammatory Pain.Biomedicines · 2026Article
- Essential Oils from Seed-Depleted Infructescences of Industrial Hemp: Chemical Diversity and Biological Potential.Molecules (Basel, Switzerland) · 2026Article
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- Clinical Potential of Essential Oils: Cytotoxicity, Selectivity Index and Antimicrobial Activity Against Gram-Negative ESKAPEE Pathogens.Antibiotics (Basel, Switzerland) · 2026Review
- Chemical composition ofFrontiers in pharmacology · 2026Article
- Penaeus monodon shell waste-derived chitosan nanoparticles show biocompatibility and broad antimicrobial activity.Scientific reports · 2025Article
- Valorization of flowers and their role in circular bioeconomy and sustainable development goals.Bioresources and bioprocessing · 2025Review
- Natural essential oils as a new therapeutic tool in colorectal cancer.Cancer cell international · 2022Review
- Bioactivity and Chemical Profile ofFoods (Basel, Switzerland) · 2022Article
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- The Effects of Tormentic Acid and Extracts fromTheScientificWorldJournal · 2021Article
- Mosquito Larvicidal Activity, Antimicrobial Activity, and Chemical Compositions of Essential Oils from Four Species of Myrtaceae from Central Vietnam.Plants (Basel, Switzerland) · 2020Article
- Proapoptotic Activity ofMolecules (Basel, Switzerland) · 2020Article
- Phytochemical profiling and seasonal variation of essential oils of three Callistemon species cultivated in Egypt.PloS one · 2019Article
- Phytochemical Constituents, Antioxidant, Cytotoxicity, Antimicrobial, Antitrypanosomal, and Antimalarial Potentials of the Crude Extracts ofEvidence-based complementary and alternative medicine : eCAM · 2019Article
- Frankincense, pine needle and geranium essential oils suppress tumor progression through the regulation of the AMPK/mTOR pathway in breast cancer.Oncology reports · 2018Article
- Terpene constituents of the aerial parts, phenolic content, antibacterial potential, free radical scavenging and antioxidant activity of Callistemon citrinus (Curtis) Skeels (Myrtaceae) from Eastern Cape Province of South Africa.BMC complementary and alternative medicine · 2017Article
- Pharmacological Potential of Phylogenetically Diverse Actinobacteria Isolated from Deep-Sea Coral Ecosystems of the Submarine Avilés Canyon in the Cantabrian Sea.Microbial ecology · 2017Article
- Himalayan Aromatic Medicinal Plants: A Review of their Ethnopharmacology, Volatile Phytochemistry, and Biological Activities.Medicines (Basel, Switzerland) · 2016Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPlant-based traditional system of medicine continues to play an important role in healthcare. In order to find new potent source of bioactive molecules, we studied the cytotoxic activity of the essential oils from the flowers and leaves of Callistemon citrinus. This is the first report on anticancer potential of essential oils of C. citrinus.
methodsCytotoxicity of essential oil was evaluated using sulfo-rhodamine B (SRB) assay against human lung carcinoma (A549), rat glioma (C-6), human colon cancer (Colo-205) and human cervical cancer (SiHa) cells. Apoptosis induction was evaluated by caspase-3/7 activity which was further confirmed by western blotting. Percentage cell apoptosis was determined by Annexin V based dead cell assay followed by DNA content as cell cycle analysis against A549 and C-6 cells. While 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to check the toxicity against normal human peripheral blood mononuclear cells (PBMCs), the immunomodulatory activity on mouse splenocytes was evaluated using SRB assay.
resultsThe GC and GC-MS analysis of these essential oils revealed high content of α-pinene (32.3%), limonene (13.1%) and α-terpineol (14.6%) in leaf sample, whereas the flower oil was dominated by 1,8-cineole (36.6%) followed by α-pinene (29.7%). The leaf oil contained higher amount of monoterpene hydrocarbons (52.1%) and sesquiterpenoids (14%) as compared to flower oil (44.6% and 1.2%, respectively). However, the flower oil was predominant in oxygenated monoterpenes (43.5%). Although both leaf and flower oils showed highest cytotoxicity on A549 cells (61.4%±5.0 and 66.7%±2.2, respectively), only 100 μg/mL flower oil was significantly active against C-6 cells (69.1%±3.1). Interestingly, no toxicity was recorded on normal cells.
conclusionHigher concentration of 1,8-cineole and/or synergistic effect of the overall composition were probably responsible for the efficacy of flower and leaf oils against the tested cells. These oils may form potential source of natural anti-cancer compounds and play important role in human health.
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